两性霉素B脂质体的制备及药动学

发布时间：2018-10-29 11:14

【摘要】：目的:制备两性霉素B脂质体并对其药动学进行研究。方法:采用有机溶剂注入法制备两性霉素B脂质体,用微射流进行整粒,用动态光散射法测定粒径,采用Ultrafree-CL离心超滤管分离游离两性霉素B(F-AMB)与复合两性霉素B(L-AMB)检测包封率,用Wistar大鼠进行药动学研究。结果:两性霉素B脂质体平均粒径为(115±20)nm;包封率99.9%;大鼠静脉注射,实验药与Amphotec汶的Cmax,AUC0~168 h和AUC0~∞无显著性差异(P0.05)。结论:采用有机溶剂注入法制备两性霉素B脂质体具有较高包封率,在大鼠体内的药动学特征与Amphotec汶一致。
[Abstract]:Objective: to prepare amphotericin B liposome and study its pharmacokinetics. Methods: amphotericin B liposomes were prepared by organic solvent injection. The entrapment efficiency of free amphotericin B (F-AMB) and compound amphotericin B (L-AMB) was determined by Ultrafree-CL centrifugation ultrafiltration tube. The pharmacokinetics of Wistar rats was studied. Results: the mean particle size of amphotericin B liposomes was (115 卤20) nm; entrapment efficiency 99.9, and there was no significant difference in Cmax,AUC0~168 h and AUC0~ 鈭，

本文编号：2297537