二甲基亚砜充当保护剂灌注兔离段肢体局部药物浓度的相对回收率

发布时间：2018-10-29 17:14

【摘要】：背景:二甲基亚砜冷冻保护剂的效果在低温医学应用中得到了大量验证,目前尚无利用微透析技术对二甲基亚砜进行检测的文献报道。目的:考察不同浓度断肢再植冷冻保护剂二甲基亚砜微透析的相对回收率。方法:应用增量法及减量法检测体外不同浓度(2%、5%、8%)二甲基亚砜微透析线性探针的相对回收率,并进行体内校正实验,以减量法估算二甲基亚砜在兔离断肢体的相对回收率。结果与结论:增量法检测2%、5%、8%浓度二甲基亚砜的相对回收率分别为(49.49±3.56)%,(46.30±1.48)%,(52.66±2.54)%,平均回收率为(49.48±3.18)%;减量法检测2%、5%、8%浓度二甲基亚砜的相对回收率分别为(50.99±6.89)%,(43.86±1.35)%,(50.67±0.75)%,平均回收率为(48.51±4.03)%,两种方法检测的二甲基亚砜微透析探针相对回收率基本相同。体内校正实验显示回收率随二甲基亚砜浓度的变化较小,平均回收率为(15.45±4.80)%。结果表明不同浓度二甲基亚砜的微透析探针相对回收率无明显差异。
[Abstract]:Background: the effect of dimethyl sulfoxide (DMSO) cryopreservation agent has been extensively verified in the application of cryogenic medicine, and there is no literature report on the detection of DMSO by microdialysis technology. Objective: to investigate the relative recovery of dimethyl sulfoxide microdialysis (DMSO) for replantation of amputated limbs with different concentrations. Methods: the relative recovery rate of dimethyl sulfoxide microdialysis probe with different concentrations (2 5% and 8%) in vitro was detected by incremental method and decrement method. The relative recovery rate of dimethyl sulfoxide (DMSO) in rabbit limb was estimated by deweighting method. Results and conclusion: the relative recoveries of dimethyl sulfoxide were (49.49 卤3.56)%, (46.30 卤1.48)%, (52.66 卤2.54)%, and the average recovery was (49.48 卤3.18)%, respectively. The relative recoveries of dimethyl sulfoxide were (50.99 卤6.89)%, (43.86 卤1.35)%, (50.67 卤0.75)%, and the average recovery was (48.51 卤4.03)%, respectively. The relative recovery of dimethyl sulfoxide microdialysis probe detected by the two methods was basically the same. The in vivo calibration test showed that the recovery rate changed slightly with the concentration of dimethyl sulfoxide, and the average recovery was (15.45 卤4.80)%. The results showed that there was no significant difference in the relative recovery of dimethyl sulfoxide (DMSO) microdialysis probe.
【作者单位】：首都医科大学附属北京朝阳医院;解放军第二炮兵总医院;
【基金】：全军十一五医药卫生科研基金项目(08G051) 国家自然科学基金项目(81301662;81341015)~~
【分类号】：R965

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