超高效液相串联质谱法测定大鼠西酞普兰及其代谢物的血药浓度

发布时间：2018-10-31 13:06

【摘要】：目的建立快速测定大鼠体内西酞普兰及其代谢物(N-氧化西酞普兰和N-去甲基西酞普兰)浓度的超高效液相串联质谱法。方法用乙酸乙酯萃取法处理血浆,色谱柱为ACQUITY UPLC BEH C18柱(50 mm×2.1 mm,1.7μm);流动相为乙腈-0.1%甲酸水,梯度洗脱,流速为0.4 m L·min-1;用正离子多离子反应监测(MRM)扫描,内标为曲马多。结果血浆中西酞普兰、N-去甲基西酞普兰和N-氧化西酞普兰的线性范围分别为0.1~10.0,0.1~10.0,0.01~2.00 ng·m L-1(r=0.999 1,0.999,0.999 6),定量下限分别为0.05,0.05,0.01ng·m L-1。其回收率分别在96.09%~105.33%。三者的日内、日间精密度均RSD8.69%。结论该方法操作简便、快捷,灵敏度高,适于大鼠体内西酞普兰及其代谢物的药代动力学研究。
[Abstract]:Objective to establish a high performance liquid chromatography tandem mass spectrometry method for the rapid determination of citalopram and its metabolites (N-oxide-citalopram and N-demethylcitalopram) in rats. Methods Plasma was treated with ethyl acetate extraction. The chromatographic column was ACQUITY UPLC BEH C18 column (50 mm 脳 2.1 mm,1.7 渭 m); mobile phase was acetonitrile-0.1% formic acid water, gradient elution, flow rate was 0.4 mL min-1;). The (MRM) scan was monitored by positive ion multiion reaction, and the internal standard was tramadol. Results the linear range of plasma citalopram, N-demethylcitalopram and N-oxide-citalopram in plasma was 0.1 ~ 10.0 ~ (10) ~ (10) ~ (-1) ~ (0.1) ~ (-1) ~ 2. 00 ng 路mL ~ (-1) (r ~ (0.999) 1 ~ (0.999) ~ (-1), respectively. The lower limit of quantification was 0.05 ~ 0.05ng / mL ~ (-1), respectively. The recoveries were 96.09 and 105.33, respectively. The intra-day and daytime precision of all three are RSD8.69%.. Conclusion the method is simple, rapid and sensitive. It is suitable for pharmacokinetics of citalopram and its metabolites in rats.
【作者单位】：丽水市人民医院药学部;丽水市人民医院临床药学实验室;温州医科大学药学院药理教研室;
【基金】：卫生部行业科研专项基金资助项目(201302008)
【分类号】：R96;O657.63

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