地佐辛对坐骨神经慢性压迫性疼痛大鼠脊髓胶质纤维酸性蛋白表达的影响

发布时间：2018-11-03 07:53

【摘要】：目的探索地佐辛对坐骨神经慢性压迫性疼痛(CCI)大鼠脊髓胶质纤维酸性蛋白(GFAP)的影响及对坐骨神经的保护作用。方法按照体重将SD大鼠随机为4组,每组8只:假手术组、模型组和低、高2个剂量实验组(地佐辛,2.5,10 mg·kg~(-1))。于手术后即刻至术后7 d,各组每天腹腔注射药物1次,假手术组和模型组给予等体积的0.9%NaCl。于给药7 d后,电镜下观察各组坐骨神经的病理形态学变化,以免疫荧光组织化学法观测各组脊髓GFAP表达。结果地佐辛连续给药7 d后,低剂量实验组大鼠的坐骨神经纤维肿胀,鞘细胞数目减少,部分髓鞘溶解;高剂量实验组大鼠有少数正常髓鞘,较模型组神经纤维肿胀减轻,但较假手术组鞘细胞减少,部分髓鞘中可见轴突。大鼠脊髓中GFAP的表达水平:假手术组、模型组、低、高2个剂量实验组分别为1.03±0.28,2.08±0.55,1.61±0.14,1.25±0.45。与假手术组比较,模型组差异有统计学意义(P0.001);与模型组比较,低、高2个剂量实验组差异均有统计学意义(P0.05,P0.01)。结论地佐辛对神经病理性疼痛的抑制作用,可能与下调脊髓GFAP的表达、影响星形胶质细胞的活化和减轻坐骨神经水肿有密切关联。
[Abstract]:Objective to investigate the effect of dizosin on glial fibrillary acidic protein (GFAP) (GFAP) and its protective effect on sciatic nerve in (CCI) rats with chronic compression pain of sciatic nerve. Methods according to body weight, SD rats were randomly divided into 4 groups with 8 rats in each group: sham-operated group, model group, low dose and high dose group (dizosin, 2.5 min 10 mg kg~ (-1). From immediately after operation to 7 days after operation, each group was intraperitoneally injected with drug once a day. The sham operation group and the model group were given 0.9 sodium chloride of the same volume. The histopathological changes of sciatic nerve in each group were observed under electron microscope and the expression of GFAP in spinal cord of each group was observed by immunofluorescence histochemical method 7 days after administration. Results after continuous administration of dizosin for 7 days, the sciatic nerve fibers in the low-dose experimental group were swollen, the number of sheath cells decreased, and some myelins were dissolved. There were a few normal myelin sheath in the high dose experimental group, which was less than that in the model group, but decreased compared with the sham operation group, and some axons were found in the myelin sheath. The expression level of GFAP in spinal cord of rats: sham operation group, model group, low dose and high dose group were 1.03 卤0.28 卤2.08 卤0.55V 1.61 卤0.141.61 卤0.141.1.25 卤0.45 respectively. Compared with sham-operation group, the difference between model group and model group was statistically significant (P0.001), compared with model group, the difference of low dose and high dose group was statistically significant (P0.05 + P0.01). Conclusion the inhibitory effect of dizosin on neuropathic pain may be related to the down-regulation of GFAP expression in spinal cord, the activation of astrocytes and the reduction of sciatic nerve edema.
【作者单位】：福建省福州儿童医院麻醉科;福建省立医院药学部;
【基金】：福州市科技计划基金资助项目(2016-S-127)
【分类号】：R965

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