体外翻转肠囊法研究纳米二氧化钛对幼年大鼠小肠葡萄糖吸收的影响

发布时间：2018-11-03 18:58

【摘要】：目的:探究纳米二氧化钛(TiO_2)对幼年大鼠小肠葡萄糖吸收功能的影响及其尺寸效应。方法:取63只幼年(4周龄)雄性Sprague-Dawley(SD)大鼠的小肠,建立63个体外翻转肠囊模型,研究葡萄糖浓度分别为10、25、50、100、200、400、800 mmol/L时,暴露于0、50 mg/L纳米TiO_2(24 nm)2 h对小肠葡萄糖吸收功能的影响;并在此基础上,继续利用体外翻转肠囊模型,在400 mmol/L葡萄糖浓度下,研究暴露于0、10、50、200 mg/L纳米TiO_2(24 nm)和微米TiO_2(120 nm)2 h对小肠葡萄糖吸收功能的影响。每组3个肠囊。结果:幼年大鼠小肠的葡萄糖累积吸收量随葡萄糖浓度和染毒时间的增加而增加,仅在400 mmol/L葡萄糖浓度下,观察到50 mg/L纳米TiO_2染毒组的葡萄糖累积吸收量和吸收速率在30 min(t_(累积吸收量)=3.254,P0.05;t吸收速率=3.958,P0.05)、90 min(t累积吸收量=3.323,P0.05;t吸收速率=3.063,P0.05)、120 min(t累积吸收量=2.834,P0.05;t吸收速率=3.002,P0.05)时显著低于对照组。第二步研究中,与对照组相比,不同剂量纳米TiO_2或微米TiO_2染毒对幼年大鼠小肠葡萄糖的累积吸收量及乳酸累积生成量无明显影响。在相同染毒时间及染毒剂量下,未观察到纳米和微米TiO_2染毒组组间葡萄糖累积吸收量及乳酸累积生成量的差异有统计学意义。结论:纳米TiO_2短期暴露可能对幼年大鼠小肠葡萄糖吸收具有一定的抑制作用,且与微米TiO_2的差异不明显。
[Abstract]:Aim: to investigate the effect of nanometer titanium dioxide (TiO_2) on glucose absorption in small intestine of young rats and its size effect. Methods: the small intestine of 63 young (4-week-old) male Sprague-Dawley (SD) rats were selected and 63 models of intestinal sac were established in vitro. The concentration of glucose was 10 ~ (25) ~ (50) ~ (100) 200400800 mmol/L, respectively. Effects of exposure to 0 ~ (50) mg/L nanometer TiO_2 (24 nm) for 2 h on glucose absorption in small intestine; On this basis, the effects of exposure to 0101050200 mg/L nanometer-sized TiO_2 (24 nm) and micron TiO_2 (120 nm) for 2 h on the absorption of glucose in the small intestine were studied by using the model of in vitro turnover of intestinal sac. At the concentration of 400 mmol/L glucose, the effects of Nano TiO_2 (24 nm) and micron TiO_2 (120 nm) were studied. There were 3 intestinal sac in each group. Results: the accumulation of glucose in the small intestine of young rats increased with the increase of glucose concentration and exposure time, only at 400 mmol/L glucose concentration. The cumulative glucose absorption and absorption rate of 50 mg/L TiO_2 exposed group were observed at 30 min (t _ (cumulative absorption) = 3.254 (P _ (0.05). T absorption rate = 3.958 (P 0.05), 90 min (t cumulative absorption rate = 3.323 (P 0.05), 120 min (t cumulative absorption rate (120 min (t) = 2.834 (P 0.05); T absorption rate was significantly lower than that of control group (P 0.05). In the second step, compared with the control group, different doses of nanometer TiO_2 or micron TiO_2 had no significant effect on the accumulative absorption of glucose and the accumulation of lactic acid in the small intestine of young rats. Under the same exposure time and dose, there was no significant difference in the cumulative glucose absorption and lactic acid production between the groups exposed to nanometre and micron TiO_2. Conclusion: the short term exposure of nanometer TiO_2 may inhibit the absorption of glucose in the small intestine of young rats, and there is no significant difference between nano TiO_2 and micron TiO_2.
【作者单位】：北京大学公共卫生学院劳动卫生与环境卫生学系;
【基金】：国家自然科学基金青年基金项目(31400863) 北京市自然科学基金资助项目(7172116)资助~~
【分类号】：R99

【相似文献】