一氧化碳发色罗非鱼片急性毒性与遗传毒性研究

发布时间：2018-11-06 15:49

【摘要】：采用小鼠急性毒性实验、鼠伤寒沙门氏菌回复突变实验(Ames)、小鼠骨髓细胞微核实验、小鼠睾丸染色体畸变实验研究发色罗非鱼片的急性毒性和遗传毒性。结果表明,小鼠按照急性经口最大耐受剂量26.4 g/kg体重给予受试物后,14 d内观察实验雌性和雄性小鼠均未出现死亡情况。遗传毒性实验表明,发色罗非鱼片50、500、5000μg的处理组TA1535、TA1537、TA1538、TA98、TA100的鼠伤寒沙门氏菌回变菌落数最大值为(165±7)个/皿,显著低于添加二硝酸氟(NF)和2-苯蒽胺(ANTH)的阳性对照组(p0.01);高、中、低剂量组小鼠骨髓微核率分别为(1.0‰±0.5‰)、(0.3‰±0.5‰)和(0.5‰±0.7‰),剂量组之间无明显的剂量关系;高、中、低剂量组睾丸染色体总畸变率分别为5.5%、5.9%和4.9%,与阴性对照组总畸变率4.8%相比无显著性差异,明显低于阳性组(p0.01)。说明本次实验条件下,发色罗非鱼片经口最大耐受剂量(MTD)15 g/kg安全剂量,Ames实验、小鼠骨髓微核实验和睾丸染色体畸变实验均呈阴性,说明CO发色罗非鱼片无急性毒性及遗传毒性。
[Abstract]:The acute toxicity and genetic toxicity of chromatic tilapia tablets were studied by mouse acute toxicity test, (Ames), mouse bone marrow micronucleus test and mouse testicular chromosome aberration test. The results showed that there was no death in both female and male mice within 14 days after the mice were given acute oral maximum tolerance dose of 26.4 g/kg. The genotoxicity test showed that the maximum number of reversion colonies of Salmonella typhimurium in TA1535,TA1537,TA1538,TA98,TA100 treated group was (165 卤7) / dish. It was significantly lower than that of the positive control group supplemented with fluorine dinitrate (NF) and 2-phenanthlamine (ANTH) (p0.01). The micronucleus rate of bone marrow in the high, middle and low dose groups was (1.0 鈥，

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