三氯异氰尿酸对斑马鱼胚胎及幼鱼的发育毒性

发布时间：2018-11-10 10:34

【摘要】：目的探讨新型消毒剂三氯异氰尿酸(TCCA)对斑马鱼胚胎及幼鱼的发育毒性。方法选择受精后2 h的斑马鱼胚胎进行染毒:①将胚胎置于含有TCCA 50,100,150,200,250和300 mg·L~(-1)的培养液中暴露48 h,检测半数致死浓度(LC_(50))。②将胚胎置于含有TCCA 0,10.4,20.8和41.7 mg·L~(-1)的培养液中暴露96 h,分别于暴露后48,72和96 h检测胚胎死亡率、畸形率和心率,采用羟胺法于暴露后2,4和6 h检测胚胎组织超氧化物歧化酶(SOD)的活性,HE染色观察暴露后7 d幼鱼头部组织病理结构。结果 TCCA暴露48 h,斑马鱼胚胎LC_(50)为166.9 mg·L~(-1)。与正常对照组相比,暴露后96 h,TCCA 41.7 mg·L~(-1)组斑马鱼胚胎死亡率和畸形率显著升高(P0.05),TCCA各剂量组心率显著下降(P0.05);暴露后72h,TCCA41.7mg·L~(-1)组死亡率显著升高(P0.05),20.8和41.7mg·L~(-1)组畸形率显著升高(P0.05),心率显著下降(P0.05);暴露后48 h,TCCA 41.7 mg·L~(-1)组畸形率显著升高(P0.05)、心率显著下降(P0.05)。TCCA暴露4和6 h,20.8和41.7 mg·L~(-1)组SOD活性较正常对照组显著下降(P0.05)。TCCA暴露7 d后,与正常对照组相比,各浓度组斑马鱼幼鱼均出现脑和眼部间隙变大及眼部视网膜分层不明显的病理改变。结论 TCCA对斑马鱼胚胎发育有毒性作用,能引起胚胎发育早期SOD活性下降,造成幼鱼视网膜组织结构损伤。
[Abstract]:Objective to study the developmental toxicity of a new disinfectant trichloroisocyanuric acid (TCCA) to zebrafish embryos and young fish. Methods zebrafish embryos were exposed to TCCA 50100150200250 and 300 mg L ~ (-1) for 48 h. The median lethal concentration (LC_ (50). 2) was detected by LC_ (50). 2). The embryo death rate, deformity rate and heart rate were measured at 4872h and 96h after exposure to the culture medium containing TCCA 010.4L ~ (-1) and 41.7 mg L ~ (-1), respectively. The activity of superoxide dismutase (SOD) in embryonic tissues was detected by hydroxylamine method at 2h and 6h after exposure. The histopathological structure of the head of young fish was observed by HE staining at 7 days after exposure. Results the LC_ (50) of zebrafish embryos was 166.9 mg L ~ (-1) after TCCA exposure for 48 h. Compared with the normal control group, the fetal mortality and abnormal rate of zebrafish were significantly increased in TCCA 41.7 mg L ~ (-1) group at 96 h after exposure (P0.05). The mortality rate of TCCA 41.7 mg L ~ (-1) group increased significantly (P0.05), the deformity rate of 20.8 and 41.7mg L ~ (-1) groups increased significantly (P0.05), and the heart rate decreased significantly (P0.05). 48 h after exposure, the deformity rate of TCCA 41.7 mg L ~ (-1) group increased significantly (P0.05), and the heart rate decreased significantly (P0.05). TCCA exposure for 4 and 6 hours). The SOD activity in 20.8 and 41.7 mg L-1 groups was significantly lower than that in the normal control group (P0.05). TCCA exposure for 7 days, compared with the normal control group). The histopathological changes of brain and eye space and retinal stratification were not obvious in juvenile zebrafish. Conclusion TCCA has a toxic effect on the embryonic development of zebrafish, which can induce the decrease of SOD activity in the early stage of embryonic development and damage the retinal tissue structure of young fish.
【作者单位】：湖南农业大学食品科学技术学院;深圳市疾病预防控制中心;
【基金】：深圳市科技研发资金项目(CYJ2014041017-1018510) 深圳市卫计委重点学科建设能力提升项目(201506064) 深圳市科技研发资金学科布局项目(JCYJ20160328144536436)~~
【分类号】：R99

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