香烟烟雾暴露对大鼠睾丸组织雄激素结合蛋白mRNA及蛋白表达水平的影响

发布时间：2018-11-22 20:38

【摘要】：目的探讨香烟烟雾暴露对大鼠睾丸结构及睾丸组织雄激素结合蛋白(ABP)mRNA及蛋白表达水平的影响。方法清洁级成年雄性SD大鼠160只,随机分为对照组和低(10支)、中(20支)、高(30支)剂量染毒组,各组大鼠分别染毒2、4、6、8、12周,共16组,每组10只。实验组采用呼吸道静式染毒,每日1次,每次30 min。观察大鼠睾丸组织结构,采用RT-PCR及Western Blot法检测睾丸组织ABP mRNA及蛋白表达水平。结果染毒组大鼠睾丸病理切片可见,生精细胞排列紊乱疏松,支持细胞呈现空泡化。大鼠睾丸组织ABP mRNA和蛋白表达水平均随染毒剂量的增加而降低,染毒4周及12周后中、高剂量组ABP基因mRNA表达水平低于对照组,染毒6周后高剂量组ABP基因mRNA表达水平低于对照组,染毒4~8周后高剂量组ABP蛋白表达水平低于对照组,染毒12周后低、中、高剂量组ABP蛋白表达水平低于对照组,差异均有统计学意义(P0.05)。结论香烟烟雾暴露可引起雄性大鼠睾丸组织受损,且睾丸组织ABP基因mRNA及蛋白的表达水平下降。
[Abstract]:Objective to investigate the effects of cigarette smoke exposure on the expression of androgen binding protein (ABP) mRNA and protein in testis of rats. Methods 160 clean grade adult male SD rats were randomly divided into control group (n = 10), medium (n = 20) and high dose (n = 30). The experimental group was treated with respiratory tract administration once a day for 30 min.. The structure of rat testis was observed and the expression of ABP mRNA and protein in testis was detected by RT-PCR and Western Blot. Results in the exposed group, testicular sections were observed, spermatogenic cells were disordered and loose, and Sertoli cells were vacuolated. The expression of ABP mRNA and protein in testis of rats decreased with the increase of exposure dose. After 4 and 12 weeks of exposure, the mRNA expression of ABP gene in high dose group was lower than that in control group. The expression level of ABP gene mRNA in high dose group was lower than that in control group after 6 weeks of exposure, the ABP protein expression level in high dose group was lower than that in control group after 8 weeks of exposure, and the expression level of ABP protein in middle and high dose group was lower than that in control group after 12 weeks of exposure. The difference was statistically significant (P0.05). Conclusion cigarette smoke exposure can damage the testis of male rats and decrease the expression of ABP gene mRNA and protein in testis.
【作者单位】：新疆医科大学公共卫生学院毒理学教研室;
【基金】：国家自然科学基金(81260433) 新疆维吾尔自治区自然科学基金(2014211C015)
【分类号】：R99

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