左氧氟沙星-N-氧化物对映体的手性分离研究
发布时间：2018-11-25 15:01
【摘要】：目的建立左氧氟沙星-N-氧化物手性分离和光学纯度检查的高效液相色谱方法。方法色谱柱采用十八烷基硅烷键和硅胶为填充剂,流动相为D-苯丙氨酸硫酸铜溶液-甲醇(80∶20),流速为1.0 m L·min-1;检测波长330 nm,柱温:40℃。结果在此条件下,左氧氟沙星-N-氧化物及其对映体能够达到基线分离,分离度为3.2。结论简单快速,重复性好,能够用于左氧氟沙星-N-氧化物对映体的分离和纯度考察。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for chiral separation and optical purity determination of levofloxacin-N-oxide. Methods the column was packed with octadecyl silane bond and silica gel. The mobile phase was D- phenylalanine copper sulfate solution-methanol (80:20). The flow rate was 1.0 mL min-1; at 330 nm, column temperature: 40 鈩，
本文编号：2356522
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for chiral separation and optical purity determination of levofloxacin-N-oxide. Methods the column was packed with octadecyl silane bond and silica gel. The mobile phase was D- phenylalanine copper sulfate solution-methanol (80:20). The flow rate was 1.0 mL min-1; at 330 nm, column temperature: 40 鈩，
本文编号：2356522
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