荧光探针法测定奥沙利铂的方法及机理研究

发布时间：2018-11-25 19:42

【摘要】：目的:建立一种简单、快速、灵敏的巴马汀荧光探针法,用于测定抗癌药物奥沙利铂。方法:以奥沙利铂和巴马汀探针对葫芦[7]脲空腔竞争为基础,巴马汀和葫芦[7]脲的水溶液本身无荧光,二者混合后体系荧光显著增强,但当加入无荧光的奥沙利铂后,体系的荧光又显著猝灭。结果:在0.05~1.75μg·m L~(-1)的范围内奥沙利铂的浓度与荧光猝灭值呈良好的线性关系,最低定量限为20 ng·m L~(-1),检测限为6 ng·m L~(-1)。并通过核磁共振和密度泛函理论对其作用机理进行了探究,证实奥沙利铂与葫芦[7]脲可以形成1∶1的稳定包合物。结论:该方法可用于血浆中奥沙利铂的测定。
[Abstract]:Objective: to establish a simple, rapid and sensitive fluorescence probe method for the determination of oxaliplatin. Methods: based on the competition of oxaliplatin and palmatine probe to the cavity of gourd [7] urea, there was no fluorescence in the aqueous solution of Bamatin and gourd [7] urea. The fluorescence of the system was also significantly quenched. Results: there was a good linear relationship between the concentration of oxaliplatin and fluorescence quenching in the range of 0.05 渭 g mL ~ (-1). The lowest quantitative limit was 20 ng mL ~ (-1) and the detection limit was 6 ng mL ~ (-1). The mechanism of its action was studied by NMR and density functional theory. It was proved that oxaliplatin and cucurbitu [7] urea could form a stable inclusion complex of 1:1. Conclusion: this method can be used for the determination of oxaliplatin in plasma.
【作者单位】：山西师范大学化学与材料科学学院;山西师范大学现代文理学院;
【基金】：国家自然科学基金项目(81402895)
【分类号】：R917

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