Nrf2-ARE信号通路在左乙拉西坦抗癫痫中的作用

发布时间：2018-11-25 20:19

【摘要】：目的探讨Nrf2-ARE信号通路在左乙拉西坦抗癫痫中的作用,以及应用左乙拉西坦对认知功能的影响。方法成年♂SD大鼠36只,250~300 g,随机分为生理盐水对照(control)组、戊四唑(1,5-pentamethylene-1H-tetrazole,PTZ)癫痫模型组、左乙拉西坦(levetiracetam,LEV)对照组以及左乙拉西坦治疗组,每组9只。以Morris水迷宫测试大鼠的空间学习记忆能力;用免疫印迹法测定海马组织中Nrf2、HO-1和NQO1蛋白表达量。结果与癫痫模型组相比,给予左乙拉西坦治疗的癫痫大鼠在Morris水迷宫测试中潜伏期明显缩短(P0.05),海马Nrf2、HO-1、NQO1蛋白表达均明显增高(P0.01)。结论左乙拉西坦能够改善癫痫大鼠的认知功能,其机制可能通过Nrf2-ARE通路,使HO-1、NQO1蛋白表达增多,起到抗癫痫的作用。
[Abstract]:Objective to investigate the role of Nrf2-ARE signaling pathway in levoethiracetam antiepileptic and the effect of levoethiracetam on cognitive function. Methods Thirty-six adult SD rats were randomly divided into three groups: normal saline control group (control), pentamethylene-1H-tetrazolezole (PTZ) model group, levoethylacetam (levetiracetam,LEV) control group and levoethoxetam treatment group. There were 9 rats in each group. The spatial learning and memory abilities of rats were measured by Morris water maze, and the expression of Nrf2,HO-1 and NQO1 proteins in hippocampus were measured by Western blot. Results compared with the epileptic model group, the latency of Morris water maze test in the epileptic rats treated with levoethylacetam was significantly shortened (P0.05), and the expression of Nrf2,HO-1,NQO1 protein in hippocampus was significantly increased (P0.01). Conclusion Levoethylacetam can improve the cognitive function of epileptic rats, and its mechanism may be through the Nrf2-ARE pathway, increase the expression of HO-1,NQO1 protein, and play an antiepileptic role.
【作者单位】：徐州医科大学麻醉学院;
【基金】：国家自然科学基金资助项目(No 81171041) 江苏省高校自然科学基金重点项目(No 13KJA320001) 江苏省自然科学基金资助项目(No BK20161171) 江苏省大学生创新创业训练计划(No 201610313039Y) 江苏高校品牌专业建设工程资助项目(No PPZY2015A066)
【分类号】：R965

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