阿扑西林的体外抗菌作用研究

发布时间：2018-12-07 15:32

【摘要】：目的评价阿扑西林的体外抗菌活性,并与目前临床常用的抗菌谱类似的6种药物进行比较,为阿扑西林在我国的临床应用及了解其抗菌特性提供依据。方法使用从南京、沈阳、广州及成都4地收集并经南京东南大学附属中大医院临床检验科分离鉴定菌株,采用琼脂二倍稀释法、液体二倍稀释法和菌落计数等测定各药物的体外抗菌活性,包括最低抑菌浓度、最低杀菌浓度、杀菌曲线等。结果阿扑西林对甲氧西林敏感葡萄球菌以及绝大部分链球菌有很强的抗菌作用,MIC范围基本在0.125~8mg/L之间,其对革兰阳性菌的抗菌作用略优于同类对照药。阿扑西林对革兰阴性菌的MIC90大多为128或128mg/L,除了流感嗜血菌,黏质沙雷菌的MIC90为16mg/L,变形菌MIC90为64mg/L。阿扑西林对厌氧菌均有很好的抗菌作用,略优于对照药,MIC50值在32~64mg/L之间。各影响因素测定结果表明,细菌接种量、培养基p H值及血清含量变化对阿扑西林的MIC值无明显影响。MBC和杀菌曲线表明,阿扑西林是浓度依赖性杀菌药物。结论阿扑西林是氨基酸衍生的β-内酰胺类半合成广谱青霉素类抗生素,是目前上市的β-内酰胺类中药效最好的抗感染药物之一,与市场同类品种比较有一定优势,市场前景值得期待。
[Abstract]:Objective to evaluate the in vitro antibacterial activity of azacillin and compare it with 6 drugs with similar antimicrobial spectrum in clinical use so as to provide evidence for clinical application and understanding of antimicrobial properties of apoxicillin in China. Methods strains collected from Nanjing, Shenyang, Guangzhou and Chengdu were isolated and identified by the Department of Clinical Laboratory, affiliated Hospital of Southeast Nanjing University of China. Agar dilution method was used. In vitro antimicrobial activity of various drugs was determined by liquid dilution method and colony count, including minimum inhibitory concentration, minimal bactericidal concentration, bactericidal curve and so on. Results Apoxicillin had a strong antibacterial effect against methicillin sensitive Staphylococcus and most of Streptococcus. The range of MIC was between 0.125~8mg/L and the antimicrobial activity against Gram-positive bacteria was slightly better than that of the control. The MIC90 of azacillin against Gram-negative bacteria was 128 or 128 mg / L, except for Haemophilus influenzae, the MIC90 of M. Shareh was 16 mg / L, and the MIC90 of Proteus was 64 mg / L. Aloxicillin had a good antibacterial effect on anaerobic bacteria and was slightly better than the control drug. The MIC50 value was between 32~64mg/L. The results showed that the changes of bacterial inoculation amount, pH value of culture medium and serum content had no significant effect on the MIC value of azacillin. MBC and bactericidal curve showed that aloxicillin was a concentration-dependent bactericidal drug. Conclusion Apoxicillin is a kind of 尾 -lactam semi-synthetic broad-spectrum penicillin antibiotics derived from amino acids. It is one of the most effective anti-infective drugs of 尾 -lactam on the market at present, and has some advantages compared with the similar varieties in the market. The market outlook is to be expected.
【作者单位】：南京工业大学药学院;江苏省药物研究所;
【分类号】：R96

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