樟芝酵素对苯丙胺致PC12细胞毒性损伤的保护作用

发布时间：2018-12-11 09:53

【摘要】：目的探讨樟芝酵素对苯丙胺致PC12细胞损伤的保护作用及其机理。方法利用PC12细胞模型,设置对照组,3.5 mmol/L苯丙胺组和3.5 mmol/L苯丙胺+樟芝酵素组。(1)通过显微镜观察细胞形态的变化;(2)通过MTT法检测细胞存活率;(3)通过Western blot分析Caspase-3的变化;(4)通过流式细胞仪检测细胞凋亡和细胞内活性氧的变化。结果 (1)苯丙胺损伤了PC12细胞的结构,导致细胞凋亡增加,樟芝酵素能够减少细胞的凋亡,提高细胞的存活率;(2)樟芝酵素减少了细胞内ROS的生成;(3)樟芝酵素组procaspase-3(35 kd)蛋白含量比苯丙胺组增多,激活分子caspase-3(17 kd)含量较苯丙胺组减少。结论樟芝酵素对苯丙胺致PC12细胞毒性损伤具有保护作用,其机理与抑制细胞线粒体凋亡信号通路以及减少细胞内氧自由基的产生密切相关。
[Abstract]:Objective to investigate the protective effect of camphor luciferase on PC12 cells induced by amphetamine and its mechanism. Methods the PC12 cell model was used to set up the control group, 3.5 mmol/L amphetamine group and 3.5 mmol/L phenylaniline camphor enzyme group. (1) the morphological changes of the cells were observed by microscope; (2) the cell survival rate was detected by MTT method. (3) the changes of Caspase-3 were analyzed by Western blot, and (4) the changes of apoptosis and intracellular reactive oxygen species were detected by flow cytometry. Results (1) the structure of PC12 cells was damaged by amphetamine, and the apoptosis was increased, and the cell apoptosis was decreased and the survival rate was improved by camphor enzyme, and (2) the production of ROS was decreased by anilaniline. (3) the protein content of procaspase-3 (35 kd) in anisodycin group was higher than that in amphetamine group, and the content of activator caspase-3 (17 kd) was lower than that in amphetamine group. Conclusion Cinnamomum camphora has protective effect on PC12 cell toxicity induced by amphetamine, and its mechanism is closely related to inhibition of mitochondrial apoptosis signaling pathway and reduction of intracellular oxygen free radical production.
【作者单位】： 暨南大学口腔医学院;暨南大学基础医学院人体解剖学系;
【基金】：国家自然科学基金项目(81470055) 暨南大学大学生创新创业训练计划项目(CX15181)
【分类号】：R96
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