农达对小鼠生精作用的影响

发布时间：2018-12-11 23:48

【摘要】：目的探讨农达(roundup,活性成分为草甘膦)对小鼠生精作用的影响。方法将24只成年SPF级雄性Balb/c小鼠随机分为阴性对照(蒸馏水)组和20、200、2 000 mg/kg农达染毒组,每组6只。采用经口灌胃方式进行染毒,染毒容量为20 ml/kg,每天1次,连续染毒30 d。测定小鼠精子数量、精子活动率和血清中睾酮(T)浓度及睾丸生精细胞的凋亡情况。结果与阴性对照组相比,200、2 000 mg/kg农达染毒组小鼠精子数量减少、精子活动度降低;各剂量农达染毒组小鼠血清睾酮浓度均降低,而睾丸生精细胞的凋亡率均升高,差异均有统计学意义(P0.05)。结论在本实验染毒时间和剂量范围内,农达对雄性Balb/c小鼠的生精功能有一定的毒性作用。
[Abstract]:Objective to investigate the effect of nongda (roundup, active ingredient glyphosate) on spermatogenesis in mice. Methods Twenty-four adult SPF grade male Balb/c mice were randomly divided into negative control (distilled water) group and Nongda exposure group (20 ~ 200g / 2000 mg/kg) with 6 mice in each group. Oral administration was carried out once a day for 20 ml/kg, for 30 days. Sperm count, sperm motility, serum testosterone (T) concentration and testicular spermatogenic cell apoptosis were measured. Results compared with the negative control group, the sperm quantity and sperm motility decreased in the 2000.000 mg/kg Nongda group. The concentration of serum testosterone decreased and the apoptosis rate of testicular spermatogenic cells increased in each dose group (P0.05). Conclusion in the range of exposure time and dose, Nongda has a certain toxic effect on spermatogenic function of male Balb/c mice.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院;山东省医学科学院山东省职业卫生与职业病防治研究院;
【基金】：山东省自然科学基金(ZR2015YL030)
【分类号】：R99

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