红细胞膜包裹PLGA纳米粒的制备和体外毒性及药物释放初探

发布时间：2018-12-12 09:57

【摘要】：目的:制备红细胞膜包裹PLGA纳米粒(RBC-NP)并进行结构表征、稳定性及体外毒性和药物释放的探究。方法:纳米沉淀法制备PLGA纳米粒(PLGA NP);脂质体挤压法制备红细胞膜和RBC-NP;Malvern ZS90测定粒径和分散系数;透射电镜(TEM)观察RBC-NP的形态。将DiOC18(3)载入RBC-NP和PLGA NP,探究二者的药物体外释放特性。采用CCK8法检测细胞的相对增殖率,比较RBC-NP与PLGA NP的细胞毒性。结果:RBC-NP与PLGA NP的粒径之差为11.1~14.2 nm,分散系数在0.131~0.155。TEM显示RBC-NP呈壳-核结构,核心为PLGA,外包裹单层红细胞膜。RBC-NP在PBS和超纯水中6 d内均保持稳定。RBC-NP具有良好体外缓释作用。0.25,0.5,1 mg·m L~(-1)3组中,RBC-NP的细胞相对增殖率均显著性高于PLGA NP(P0.01)。结论:具有壳-核结构的RBC-NP已被成功制备,具有药物缓释作用且体外毒性显著降低。
[Abstract]:Aim: to prepare erythrocyte membrane coated PLGA nanoparticles (RBC-NP) and investigate the structure stability in vitro toxicity and drug release. Methods: PLGA nanoparticles (PLGA NP); liposome were prepared by nano-precipitation method, erythrocyte membrane was prepared by extrusion method, particle size and dispersion coefficient were measured by RBC-NP;Malvern ZS90, and morphology of RBC-NP was observed by transmission electron microscope (TEM). DiOC18 (3) was loaded into RBC-NP and PLGA NP, to explore the drug release characteristics in vitro. The relative proliferation rate of cells was measured by CCK8 assay, and the cytotoxicity of RBC-NP and PLGA NP was compared. Results: the difference of particle size between RBC-NP and PLGA NP was 11.1 ~ 14.2 nm, dispersion coefficient. In 0.131~0.155.TEM, RBC-NP showed a shell core structure, and the core was PLGA,. RBC-NP remained stable in PBS and ultrapure water for 6 days. RBC-NP had a good sustained release in vitro. The relative proliferation rate of RBC-NP was significantly higher than that of PLGA NP (P0.01). Conclusion: RBC-NP with shell-core structure has been successfully prepared and has drug release and in vitro toxicity is significantly reduced.
【作者单位】：上海交通大学药学院;
【基金】：国家自然科学基金资助项目(81573617) 国家重大新药创制科技重大专项资助项目(2017ZX09101005-008-002) 上海交通大学晨星学者奖励计划资助项目(B类,14X100010061)
【分类号】：R943

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