HPLC法测定维生素A在健康受试者及肝硬化患者体内的血药浓度

发布时间：2018-12-12 13:29

【摘要】：目的:建立测定人血清中维生素A(Vit A)浓度的方法,并将其应用于健康受试者及肝硬化患者。方法:血清样品经液-液萃取后,采用高效液相色谱法测定。以维生素A醋酸酯为内标,色谱柱为Kromasil C_(18),流动相为甲醇-水(98∶2,V/V),流速为1ml/min,检测波长为325 nm,柱温为室温,进样量为20μl。结果:Vit A血药浓度在0.012 4~3.210μg/ml范围内线性关系良好(r=0.997 2,n=5),定量下限为0.012 4μg/ml,日内、日间RSD为1.66%~2.97%,加样回收率为98.18%~99.56%,提取回收率为89.59%~91.38%。采用该法测得24例健康受试者和24例肝硬化患者Vit A的平均血药浓度分别为(0.71±0.08)、(0.28±0.06)μg/ml;不同年龄段健康受试者与肝硬化患者Vit A平均血药浓度比较,差异均有统计学意义(P0.05)。结论:该方法简便、快速,且灵敏度高、准确性好,可用于健康受试者及肝硬化患者血清中Vit A浓度的测定。
[Abstract]:Aim: to establish a method for the determination of vitamin A (Vit A) in human serum and to apply it to healthy subjects and patients with liver cirrhosis. Methods: the serum samples were extracted by liquid-liquid and determined by high-performance liquid chromatography. Using vitamin A acetate as internal standard, the chromatographic column was Kromasil C _ (18), the mobile phase was methanol-water (98: 2) V / V, the flow rate was 1 ml / min, the detection wavelength was 325 nm, column temperature at room temperature, the sample amount was 20 渭 L. Results the serum concentration of: Vit A was linear in the range of 0.012 ~ 3.210 渭 g/ml (r = 0.997 ~ (2), the lower limit of quantification was 0.012 4 渭 g / ml, and the daytime RSD was 1.66 ~ 2.97 渭 g 路ml ~ (-1). The recovery rate was 98.18% and 99.56%, and the recovery rate was 89.59% and 91.38%, respectively. The mean serum concentration of Vit A was (0.71 卤0.08) 渭 g / ml in 24 healthy subjects and (0.28 卤0.06) 渭 g / ml in 24 patients with liver cirrhosis, respectively. There were significant differences in mean Vit A concentrations between healthy subjects of different age groups and patients with liver cirrhosis (P0.05). Conclusion: the method is simple, rapid, sensitive and accurate. It can be used for the determination of serum Vit A in healthy subjects and patients with liver cirrhosis.
【作者单位】：天津市第三中心医院药剂科;天津市第三中心医院/天津市人工细胞重点实验室;天津市第二人民医院/天津市肝病研究所;
【基金】：天津市卫生行业重点攻关项目(No.13KG110、14KG109) 天津市卫生局科技基金项目(No.2015KY02) 王宝恩肝纤维化研究基金科研课题(No.CFHPC20151026)
【分类号】：R969.4

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