海南红树林根际糖多胞菌菌株YIM M13568的红霉素类抗生素的分离和鉴定
发布时间：2018-12-12 14:04
【摘要】：目的从分离自海南红树林根际糖多胞菌Saccharopolyspora sp.YIM M13568菌株中分离和鉴定红霉素类抗菌活性产物。方法采用反相硅胶色谱、凝胶色谱和正相硅胶色谱等技术,对菌株YIM M13568发酵产物进行分离纯化;通过1D、2D NMR和HR-ESI-MS进行结构测定。结果鉴定了3个红霉素家族抗生素的结构,包括anhydroerythromycin A(1)、3-O-mycarosylerythronolide B(2)和一个新的红霉素类化合物命名为sporeamicin D(3),并测试了它们的抗菌活性。结论糖多胞菌YIM M13568菌株是红霉素类抗生素的新资源微生物。
[Abstract]:Objective to isolate and identify erythromycin active products from Saccharopolyspora sp.YIM M13568 isolated from Hainan mangrove rhizosphere. Methods the fermentation products of YIM M13568 were isolated and purified by reverse phase silica gel chromatography, gel chromatography and normal phase silica gel chromatography, and the structure was determined by 1DX 2D NMR and HR-ESI-MS. Results the structures of three erythromycin family antibiotics, including anhydroerythromycin A (1), 3-O-mycarosylerythronolide B (2) and a new erythromycin compound named sporeamicin D (3, were identified and their antibacterial activities were tested. Conclusion YIM M 13568 is a new resource microorganism for erythromycin antibiotics.
【作者单位】： 化学生物学教育部重点实验室山东大学药学院;
【基金】：国家自然科学基金面上项目(No.81673317)
【分类号】：R915
本文编号：2374708
[Abstract]:Objective to isolate and identify erythromycin active products from Saccharopolyspora sp.YIM M13568 isolated from Hainan mangrove rhizosphere. Methods the fermentation products of YIM M13568 were isolated and purified by reverse phase silica gel chromatography, gel chromatography and normal phase silica gel chromatography, and the structure was determined by 1DX 2D NMR and HR-ESI-MS. Results the structures of three erythromycin family antibiotics, including anhydroerythromycin A (1), 3-O-mycarosylerythronolide B (2) and a new erythromycin compound named sporeamicin D (3, were identified and their antibacterial activities were tested. Conclusion YIM M 13568 is a new resource microorganism for erythromycin antibiotics.
【作者单位】： 化学生物学教育部重点实验室山东大学药学院;
【基金】：国家自然科学基金面上项目(No.81673317)
【分类号】：R915
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