GC-MS法测定甲磺酸培氟沙星中甲磺酸酯杂质的研究

发布时间：2018-12-13 14:16

【摘要】：目的建立气相质谱方法的同时测定甲磺酸培氟沙星中甲磺酸甲酯(MMS)、甲磺酸乙酯(EMS)和甲磺酸异丙酯(IMS)含量。方法经碘代试剂的柱前衍生化,将3种甲磺酸酯衍生成碘甲烷,碘乙烷和碘代异丙烷,利用DB-Wax石英毛细管柱(30m×0.25mm,0.25μm)分离,质谱检测器检测。结果 MMS、EMS和IMS分别在34~549、31~499和26~411ng/m L具有良好的线性(r≥0.9999),平均回收率分别为101.8%、102.9%和100.6%,定量限≤2.6ng/m L。结论该方法可用于甲磺酸培氟沙星中甲磺酸酯类杂质的分离分析,该方法简便、准确且快速。
[Abstract]:Objective to establish a method for simultaneous determination of methyl methanesulfonate (MMS),) ethyl methanesulfonate (EMS) and isopropyl mesosulfonate (IMS) in pefloxacin mesylate by gas phase mass spectrometry. Methods three kinds of methanesulfonate were derived into methane iodide, ethane and isopropane by pre-column derivatization of iodide reagent, and separated by DB-Wax quartz capillary column (30m 脳 0.25mm ~ 0.25 渭 m) and detected by mass spectrometry. Results MMS,EMS and IMS had a good linearity (r 鈮，

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