SPG膜乳化法制备粒径均一可控的载蛋白缓释微球
发布时间：2018-12-24 12:41
【摘要】：目的:研究一种制备粒径均一可控的载蛋白缓释微球的新工艺,探究微球粒径、载体材料、形态结构与微球载药释药性能的关系。方法:以牛血清白蛋白(BSA)为模型药物,PLGA和PEG-PLGA作为载体材料,采用SPG膜乳化法,通过调整不同孔径(3,5,7,12μm)的SPG膜制备不同粒径的微球。考察微球粒径、包封率、释放行为、表面/内部形态等性质,并对微球微观结构相关的参数如孔径、平面孔隙率等进行定量分析。结果:微球的粒径与SPG膜孔径呈正相关关系,且相关系数0.9。随着微球粒径的增大,载药量和包封率也呈现增大的趋势,突释减轻。PLGA和PEG-PLGA微球的内部结构随微球粒径增加的变化差异较大。结论:获得较为满意的制备载蛋白微球的新工艺,微球形态圆整,粒径均一可控。
[Abstract]:Aim: to study a new process for preparation of protein-loaded sustained-release microspheres with uniform and controllable particle size, and to explore the relationship between the particle size, carrier material, morphology and drug delivery properties of microspheres. Methods: bovine serum albumin (BSA) (BSA) was used as model drug, PLGA and PEG-PLGA were used as carrier materials, and SPG membrane emulsification method was used to prepare different diameter microspheres of SPG membrane with different pore size (3 ~ 5 ~ 712 渭 m). The particle size, encapsulation efficiency, release behavior, surface / internal morphology of the microspheres were investigated. The parameters related to the microstructure of the microspheres, such as pore size and planar porosity, were analyzed quantitatively. Results: the diameter of microspheres was positively correlated with the pore size of SPG membrane, and the correlation coefficient was 0.9. With the increase of the particle size of the microspheres, the drug loading and entrapment efficiency increased, and the sudden release decreased. The internal structure of PLGA and PEG-PLGA microspheres varied greatly with the increase of the particle size. Conclusion: the new technology of preparing protein loaded microspheres is satisfactory. The microspheres are round in shape and uniform in size.
【作者单位】： 中山大学药学院;中山大学附属第三医院药剂科;
【分类号】：R943
，
本文编号：2390640
[Abstract]:Aim: to study a new process for preparation of protein-loaded sustained-release microspheres with uniform and controllable particle size, and to explore the relationship between the particle size, carrier material, morphology and drug delivery properties of microspheres. Methods: bovine serum albumin (BSA) (BSA) was used as model drug, PLGA and PEG-PLGA were used as carrier materials, and SPG membrane emulsification method was used to prepare different diameter microspheres of SPG membrane with different pore size (3 ~ 5 ~ 712 渭 m). The particle size, encapsulation efficiency, release behavior, surface / internal morphology of the microspheres were investigated. The parameters related to the microstructure of the microspheres, such as pore size and planar porosity, were analyzed quantitatively. Results: the diameter of microspheres was positively correlated with the pore size of SPG membrane, and the correlation coefficient was 0.9. With the increase of the particle size of the microspheres, the drug loading and entrapment efficiency increased, and the sudden release decreased. The internal structure of PLGA and PEG-PLGA microspheres varied greatly with the increase of the particle size. Conclusion: the new technology of preparing protein loaded microspheres is satisfactory. The microspheres are round in shape and uniform in size.
【作者单位】： 中山大学药学院;中山大学附属第三医院药剂科;
【分类号】：R943
，
本文编号：2390640
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2390640.html
最近更新
教材专著
