γ-氨基丁酸改善高脂膳食小鼠的骨性能

发布时间：2018-12-30 19:14

【摘要】：探讨了高脂膳食小鼠补充γ-氨基丁酸(GABA)对骨性能的影响。40只6周龄C57BL/6雄性小鼠,随机分成4组:正常日粮组(control),高脂日粮组(HFD),高脂日粮小鼠饮水补充0.06、0.12g/dL GABA组。试验结束前一周收集各组小鼠尿液,测定钙及羟脯氨酸含量;第20周结束试验,测定小鼠股骨、胫骨参数,以及股骨氧化还原指标,荧光定量PCR检测相关基因表达。结果显示,与对照组相比,HFD组股骨出现氧化应激,股骨GAD65显著下调、GABAbR显著上调(P0.05),GSK3β显著上调而Nrf2显著下调(P0.05);股骨和胫骨钙含量、骨强度等相关性能指标显著降低,尿钙及羟脯氨酸含量显著增高,且成骨细胞分化标志基因BGLAP2、col1a1显著下调,破骨细胞分化标志基因RANKL/OPG、CTSK、TRAP显著上调(P0.05)。补充0.06、0.12g/dLGABA可恢复GABAbR、GSK3β、Nrf2表达(P0.05),减轻股骨氧化损伤,同时平衡骨细胞分化及代谢,提高股骨和胫骨性能。结果表明,GABA可调节股骨氧化还原状态,平衡骨代谢,改善股骨及胫骨性能;0.12g/dL GABA改善股骨氧化应激和股骨性能的作用优于0.06g/dL的。
[Abstract]:The effects of (GABA) supplementation on bone properties in high-fat diet mice were studied. Forty 6-week-old C57BL/6 male mice were randomly divided into four groups: normal diet group, (control), high-fat diet group, (HFD), group. Mice fed a high fat diet supplemented with 0. 06 g / dL GABA. The urine of each group was collected one week before the end of the experiment to determine the contents of calcium and hydroxyproline, the parameters of femur and tibia and the index of redox of femur were measured at the end of the 20th week, and the expression of related genes was detected by fluorescence quantitative PCR. The results showed that, compared with the control group, oxidative stress appeared in the femur of HFD group, GAD65 of femur decreased significantly, GABAbR increased significantly (P0.05), GSK3 尾 upregulated significantly and Nrf2 decreased significantly (P0.05). The contents of calcium in femur and tibia, bone strength, urine calcium and hydroxyproline were significantly decreased, and the osteoblast differentiation marker gene BGLAP2,col1a1 was significantly down-regulated, while the osteoclast differentiation marker gene RANKL/OPG,CTSK, was significantly down-regulated. TRAP was significantly up-regulated (P0.05). The supplementation of 0.06 ~ 0.12 g / d GABA could restore the expression of GABAbR,GSK3 尾 and Nrf2 (P0.05), alleviate the oxidative injury of femur, balance the differentiation and metabolism of bone cells, and improve the performance of femur and tibia. The results showed that GABA could regulate the redox state of femur, balance bone metabolism and improve the performance of femur and tibia, and that 0.12g/dL GABA could improve the oxidative stress and performance of femur better than that of 0.06g/dL.
【作者单位】：江南大学食品学院;食品科学与技术国家重点实验室江南大学;
【基金】：国家“十二五”科技支撑计划项目(2012BAD33B05) 江苏高校优势学科建设工程资助项目
【分类号】：R965

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