刺五加内生放线菌Streptomyces tauricus CWJ-107活性次级代谢产物研究
发布时间：2018-12-31 18:11
【摘要】：目的从药用植物刺五加内生放线菌Streptomyces tauricus CWJ-107次级代谢产物中发现抗耐甲氧西林金黄色葡萄球菌(MRSA)活性良好的天然产物。方法采用16S r RNA基因序列分析对菌株CWJ-107进行初步分类鉴定,大量发酵获得粗提物,通过硅胶柱层析、LH20凝胶柱层析、薄层色谱以及高效液相色谱等方法,以抗MRSA活性导向进行目标化合物的分离纯化,利用核磁共振和质谱鉴定化合物结构,并利用微孔板法测定化合物最小抑菌浓度(minimal inhibitory concentration,MIC),对其抗MRSA活性进行评价。结果菌种鉴定结果显示菌株CWJ-107为Streptomyces tauricus,从菌株CWJ-107的菌丝体中分离得到1个抗MRSA活性化合物107-A,经波谱解析证实其结构为糖基化蒽醌类化合物cinerubin B,抗菌活性测定结果证实其具有良好的抗MRSA活性,MIC值为1μg/m L。结论从刺五加内生放线菌CWJ-107的代谢产物中分离到的107-A经结构鉴定为糖基化蒽醌类化合物cinerubin B,除此之外,本文首次报道了该化合物的抗MRSA活性。
[Abstract]:Objective to find a natural product with good (MRSA) activity against methicillin-resistant Staphylococcus aureus from the secondary metabolites of endophytic actinomycetes Streptomyces tauricus CWJ-107 of Acanthopanax senticosus (Acanthopanax senticosus). Methods 16s r RNA gene sequence analysis was used to classify and identify the strain CWJ-107, and a large number of crude extracts were obtained by fermentation. The crude extracts were obtained by silica gel column chromatography, LH20 gel column chromatography, thin layer chromatography and high performance liquid chromatography. The target compounds were separated and purified by MRSA activity orientation. The structures of the compounds were identified by NMR and MS, and the minimum inhibitory concentration (minimal inhibitory concentration,MIC) of the compounds was determined by microporous plate method. The anti-MRSA activity of the compounds was evaluated. Results the strain CWJ-107 was isolated from the mycelia of CWJ-107 by Streptomyces tauricus, and a compound of anti-MRSA activity 107-A was isolated from the mycelia of CWJ-107. The structure of the compound was identified by spectral analysis as cinerubin B, a glycosylanthraquinone compound. The results of antimicrobial activity showed that it had a good MRSA activity, and the MIC value was 1 渭 g / m 路L ~ (-1) 路m ~ (-1) 路L ~ (-1). Conclusion 107-A isolated from the metabolites of actinomyces senticosus CWJ-107 was identified as cinerubin B by structure. In addition, the anti-MRSA activity of the compound was reported for the first time.
【作者单位】： 中国医学科学院北京协和医学院医药生物技术研究所;华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室;
【基金】：医药生物技术研究所院所长基金(No.2016ZX350045和No.2016ZX350053) 河北省自然科学基金资助项目(No.C2014209137)
【分类号】：R915
本文编号：2396935
[Abstract]:Objective to find a natural product with good (MRSA) activity against methicillin-resistant Staphylococcus aureus from the secondary metabolites of endophytic actinomycetes Streptomyces tauricus CWJ-107 of Acanthopanax senticosus (Acanthopanax senticosus). Methods 16s r RNA gene sequence analysis was used to classify and identify the strain CWJ-107, and a large number of crude extracts were obtained by fermentation. The crude extracts were obtained by silica gel column chromatography, LH20 gel column chromatography, thin layer chromatography and high performance liquid chromatography. The target compounds were separated and purified by MRSA activity orientation. The structures of the compounds were identified by NMR and MS, and the minimum inhibitory concentration (minimal inhibitory concentration,MIC) of the compounds was determined by microporous plate method. The anti-MRSA activity of the compounds was evaluated. Results the strain CWJ-107 was isolated from the mycelia of CWJ-107 by Streptomyces tauricus, and a compound of anti-MRSA activity 107-A was isolated from the mycelia of CWJ-107. The structure of the compound was identified by spectral analysis as cinerubin B, a glycosylanthraquinone compound. The results of antimicrobial activity showed that it had a good MRSA activity, and the MIC value was 1 渭 g / m 路L ~ (-1) 路m ~ (-1) 路L ~ (-1). Conclusion 107-A isolated from the metabolites of actinomyces senticosus CWJ-107 was identified as cinerubin B by structure. In addition, the anti-MRSA activity of the compound was reported for the first time.
【作者单位】： 中国医学科学院北京协和医学院医药生物技术研究所;华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室;
【基金】：医药生物技术研究所院所长基金(No.2016ZX350045和No.2016ZX350053) 河北省自然科学基金资助项目(No.C2014209137)
【分类号】：R915
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1 李静;海南东寨港红树林植物内生放线菌药用资源勘探[D];北京协和医学院;2016年
，本文编号：2396935
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