镰刀菌Fusariumsp sp.JN 158色素的鉴定及其抗肿瘤活性

发布时间：2019-01-08 08:57

【摘要】：通过对镰刀菌发酵所产色素的分离及结构的分析,筛选其抗肿瘤的活性成分。采用形态与分子生物学方法对镰刀菌株鉴定,利用HPLC的对该菌株发酵所产的色素进行分离,分离的产物用紫外、红外、核磁进行结构鉴定,使用MTT的方法筛选抗肿瘤的活性成分。结果表明:该镰刀菌所产色素共6个不同的峰,相对分子质量均在380左右,其中VI号峰结构新颖,为花青苷类色素,分子式为C17H18O10,能抑制A539、MCF-7、MKN-45、Hep G2、SW620五种不同肿瘤细胞的增殖,并呈现浓度的依赖性,其中MCF-7乳腺癌细胞抑制最明显(P0.01),故VI号峰色素可能是一种很有前景的防治乳腺癌的化合物。
[Abstract]:The antitumor active components of Fusarium mongolicum were screened by isolation and structure analysis of pigment produced by fermentation. The Fusarium strains were identified by morphological and molecular biological methods. The pigment produced by the fermentation was separated by HPLC. The isolated products were characterized by UV, IR and NMR. MTT method was used to screen the anti-tumor active components. The results showed that the pigment produced by Fusarium mongolicum had six different peaks, and the relative molecular weight was about 380.The peak of VI was novel, which was anthocyanin pigment, and its molecular formula was C17H18O10, which could inhibit A539MF-7MKN-45 Hep G2. The proliferation of five different tumor cells of SW620 is in a concentration-dependent manner, and the inhibition of MCF-7 breast cancer cells is the most obvious (P0.01), so VI peak pigment may be a promising compound for the prevention and treatment of breast cancer.
【作者单位】：江南大学工业生物技术教育部重点实验室;江西中医药大学基础医学院;
【基金】：国家自然科学基金项目(8116031) 国家级大学生创新创业项目(2013)
【分类号】：R915

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