毛细管电泳法测定L-谷氨酰胺呱仑酸钠颗粒中2种主成分的含量

发布时间：2019-01-09 13:26

【摘要】：目的:建立同时测定L-谷氨酰胺呱仑酸钠颗粒中2种主成分含量的方法。方法:采用毛细管电泳法。分离柱为未涂层熔融石英毛细管柱,运行缓冲液为40 mmol/L硼砂缓冲液(p H=9.22),样品缓冲液为4 mmol/L硼酸缓冲液(p H=6.97),温度为20℃;进样方式为压力进样,进样压力为0.5 psi,进样时间为5 s,分离电压为15 kV,检测波长为220 nm。结果:L-谷氨酰胺和呱仑酸钠质量浓度分别在0.332 5~13.30 mg/ml(r=0.999 3)和1.00~40.0μg/ml(r=0.998 3)范围内与各自峰面积呈良好的线性关系;精密度、稳定性试验的RSD≤2.83%;平均加样回收率分别为98.33%(RSD=4.50%,n=9)和100.03%(RSD=4.98%,n=9)。结论:该方法操作简便,结果灵敏、准确性高,可用于L-谷氨酰胺呱仑酸钠颗粒中2种主成分的含量测定。
[Abstract]:Objective: to establish a method for simultaneous determination of two principal components in L-glutamine-gluconate sodium granules. Methods: capillary electrophoresis was used. The separation column is an uncoated fused quartz capillary column, the operating buffer is 40 mmol/L borax buffer (pH 9.22), the sample buffer is 4 mmol/L boric acid buffer (pH 6.97), and the temperature is 20 鈩，

本文编号：2405685