吡咯喹啉醌对Bmi-1基因缺失引起小鼠皮肤早衰的保护作用
发布时间：2019-01-22 12:32
【摘要】：目的:研究吡咯喹啉醌(pyrroloquinoline quinone,PQQ)是否通过抑制氧化应激发挥对Bmi-1缺失小鼠引起皮肤早衰的治疗作用。方法:将同窝的Bmi-1杂合子(Bmi-1~(+/-))雌雄小鼠进行配对,取7周龄正常饮食野生型(wild type,WT)小鼠10只,正常饮食Bmi-1基因敲除(Bmi-1 knock out,BKO)小鼠10只,以及正常饮食添加PQQ的BKO小鼠(PQQ+BKO)10只,利用HE染色、组织化学、免疫组织化学及流式细胞仪检测等方法进行相关实验。结果:与BKO组小鼠比较,PQQ+BKO组小鼠整体表型部分纠正,毛色光滑,体重增加,生存期明显延长;PQQ+BKO组小鼠皮肤厚度[(142.65±0.25)μm]与BKO组小鼠[(78.45±0.35)μm]相比,差异有统计学意义(P0.01);PQQ+BKO组小鼠皮肤总胶原阳性面积百分比[(25.64±0.28)%]与BKO组小鼠[(14.87±0.47)%]相比,差异有统计学意义(P0.01);PQQ+BKO组小鼠皮肤PCNA阳性细胞百分比[(18.54±0.37)%]与BKO组小鼠[(5.85±0.64)%]相比,差异有统计学意义(P0.01);PQQ+BKO组小鼠皮肤纤维化阳性面积百分比[(48.64±0.28)%]与BKO组小鼠[(87.64±0.46)%]相比,差异有统计学意义(P0.01);PQQ+BKO组小鼠皮肤的ROS水平(298.17±0.25)较BKO组小鼠(427.80±0.63)显著下降(P0.01)。结论:PQQ通过增加皮肤胶原蛋白、促进皮肤细胞增殖、减少皮肤组织纤维化、清除氧自由基ROS水平等对Bmi-1缺失所致皮肤早衰发挥保护作用。
[Abstract]:Aim: to investigate whether pyrroloquinoline quinone (pyrroloquinoline quinone,PQQ) can inhibit oxidative stress in the treatment of premature skin failure induced by Bmi-1 deficiency in mice. Methods: Bmi-1 heterozygotes (Bmi-1~ (/ -) in the same nest were paired in 10 wild type,WT mice of 7 weeks of normal diet. Bmi-1 knock out, gene knockout of normal diet was carried out in 10 male and female mice. 10 BKO mice and 10 BKO mice fed normal diet with PQQ were tested by HE staining, histochemistry, immunohistochemistry and flow cytometry. Results: compared with BKO group, the whole phenotype of, PQQ BKO group was partly corrected, the coat color was smooth, the weight increased and the survival time was prolonged. The skin thickness of PQQ BKO group [(142.65 卤0.25) 渭 m] was significantly higher than that of BKO group [(78.45 卤0.35) 渭 m] (P0.01). The percentage of total collagen positive area in PQQ BKO group [(25.64 卤0.28)%] was significantly higher than that in BKO group [(14.87 卤0.47)%] (P0.01). The percentage of PCNA positive cells in skin of PQQ BKO group [(18.54 卤0.37)%] was significantly higher than that of BKO group [(5.85 卤0.64)%] (P0.01). The positive area of skin fibrosis in PQQ BKO group [(48.64 卤0.28)%] was significantly higher than that in BKO group [(87.64 卤0.46)%] (P0.01). The level of ROS in PQQ BKO group (298.17 卤0. 25) was significantly lower than that in BKO group (427.80 卤0. 63) (P0. 01). Conclusion: PQQ has protective effect on premature skin failure caused by Bmi-1 deficiency by increasing skin collagen, promoting skin cell proliferation, reducing skin fibrosis and eliminating ROS level of oxygen free radical.
【作者单位】： 湖南医药学院口腔医学系;南京医科大学解剖医学系骨与干细胞研究中心;南京医科大学附属口腔医院口腔种植中心;
【基金】：湖南省教育厅青年科学研究项目(14B141)
【分类号】：R965
[Abstract]:Aim: to investigate whether pyrroloquinoline quinone (pyrroloquinoline quinone,PQQ) can inhibit oxidative stress in the treatment of premature skin failure induced by Bmi-1 deficiency in mice. Methods: Bmi-1 heterozygotes (Bmi-1~ (/ -) in the same nest were paired in 10 wild type,WT mice of 7 weeks of normal diet. Bmi-1 knock out, gene knockout of normal diet was carried out in 10 male and female mice. 10 BKO mice and 10 BKO mice fed normal diet with PQQ were tested by HE staining, histochemistry, immunohistochemistry and flow cytometry. Results: compared with BKO group, the whole phenotype of, PQQ BKO group was partly corrected, the coat color was smooth, the weight increased and the survival time was prolonged. The skin thickness of PQQ BKO group [(142.65 卤0.25) 渭 m] was significantly higher than that of BKO group [(78.45 卤0.35) 渭 m] (P0.01). The percentage of total collagen positive area in PQQ BKO group [(25.64 卤0.28)%] was significantly higher than that in BKO group [(14.87 卤0.47)%] (P0.01). The percentage of PCNA positive cells in skin of PQQ BKO group [(18.54 卤0.37)%] was significantly higher than that of BKO group [(5.85 卤0.64)%] (P0.01). The positive area of skin fibrosis in PQQ BKO group [(48.64 卤0.28)%] was significantly higher than that in BKO group [(87.64 卤0.46)%] (P0.01). The level of ROS in PQQ BKO group (298.17 卤0. 25) was significantly lower than that in BKO group (427.80 卤0. 63) (P0. 01). Conclusion: PQQ has protective effect on premature skin failure caused by Bmi-1 deficiency by increasing skin collagen, promoting skin cell proliferation, reducing skin fibrosis and eliminating ROS level of oxygen free radical.
【作者单位】： 湖南医药学院口腔医学系;南京医科大学解剖医学系骨与干细胞研究中心;南京医科大学附属口腔医院口腔种植中心;
【基金】：湖南省教育厅青年科学研究项目(14B141)
【分类号】：R965
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