拉曼光谱法用于牛磺酸片鉴别及含量测定

发布时间：2019-01-23 21:25

【摘要】：目的:建立拉曼光谱法鉴别和测定牛磺酸片,并与药典方法比较。方法:以牛磺酸片作为研究对象,用水溶液提取其主成分并过滤,用拉曼光谱仪对样品溶液进行鉴别和含量测定。测定条件:激发波长780 nm,激光强度120 mW,光圈50μm,聚焦高度6.0 mm,分辨率1.0 cm~(-1),曝光时间60 s,曝光次数3次,扫描范围0~3 500 cm~(-1)。结果:牛磺酸质量浓度在5~80 mg·mL~(-1)范围内与拉曼峰面积呈良好线性关系,线性回归方程为Y=123.48X+142.51,相关系数为0.999 2(n=6);低、中、高3个浓度的平均回收率分别为97.9%、99.5%、100.3%,RSD分别为1.2%、1.7%、0.9%,6个批次样品牛磺酸含量测定结果分别为标示量的99.6%、97.5%、98.7%、98.4%、96.6%、101.5%,与2015年版中国药典标准收载的电位滴定方法测定结果基本一致。结论:本方法操作简单,重复性好,经方法验证,拉曼光谱法可以鉴别牛磺酸片,并能准确测定片剂中的牛磺酸含量,可作为牛磺酸片质量控制的分析方法。
[Abstract]:Objective: to establish a Raman spectrometric method for the identification and determination of taurine tablets and compare with pharmacopoeia method. Methods: taurine tablets were used as the object of study. The principal components of taurine tablets were extracted and filtered by aqueous solution. The samples were identified and determined by Raman spectrometer. The measurement conditions were as follows: excitation wavelength 780 nm, laser intensity 120 mW, aperture 50 渭 m, focusing height 6.0 mm, resolution 1.0 cm~ (-1), exposure time 60 s, exposure times 3 times, scanning range 0 ~ 3 500 cm~ (-1). Results: there was a good linear relationship between the concentration of taurine and Raman peak area in the range of 5o 80 mg mL~ (-1). The linear regression equation was YC 123.48X 142.51, and the correlation coefficient was 0.999 2 (NW 6). The average recoveries of three concentrations of low, medium and high concentrations were 97.9% and 99.5%, respectively. The RSD of 100.3% was 1.2 and 1.70.95, respectively. The taurine content of the six batches of samples was determined as 99.6% and 97.5% of the marked amount, and 98.7% and 98.4%, 96.6% and 101.5%, respectively. The results are in good agreement with the potentiometric titration method of Chinese Pharmacopoeia Standard 2015. Conclusion: the method is simple and reproducible. Raman spectroscopy can be used to identify taurine tablets and determine the content of taurine accurately. It can be used as an analytical method for the quality control of taurine tablets.
【作者单位】：厦门华厦学院厦门市食品药品安全重点实验室;厦门市食品药品质量检验研究院;
【基金】：国家星火计划项目(2015GA721002)
【分类号】：R927

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