津力达联合通心络对高糖诱导胰岛微血管内皮细胞损伤干预作用及机制研究

发布时间：2019-01-27 18:08

【摘要】：目的研究津力达联合通心络对高糖诱导胰岛微血管内皮细胞(MS-1)损伤的干预作用,并初步探讨其机制。方法通过MTS法检测细胞存活率筛选药物作用浓度;将细胞分为正常组(Normal)、模型组(Model)、津力达组(JLD)、通心络组(TXL)及津力达+通心络组(JLD+TXL),运用高糖(50 mmol·L-1)培养基建立损伤模型并给予药物干预,MTS法分别检测药物干预48 h、1周、2周后细胞存活率,测定给药48 h细胞上清中一氧化氮(NO)、内皮素-1(ET-1)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量,流式细胞仪检测细胞活性氧簇(ROS)水平。结果首先确定JLD 200mg·L-1、TXL 100 mg·L-1作为给药浓度。与模型组比较,给药组升高48 h、1周、2周细胞存活率及细胞上清中NO含量(P0.05,P0.01),JLD、JLD+TXL组细胞上清液ET-1含量降低(P0.05,P0.01),JLD组细胞上清液SOD含量升高(P0.05),JLD+TXL组细胞上清液MDA含量降低(P0.05);给药组细胞内ROS水平均降低(P0.05,P0.01)。结论津力达联合通心络能够改善高糖诱导MS-1细胞内皮功能损伤,并通过减轻氧化应激损伤发挥细胞保护作用。
[Abstract]:Objective to study the effect of Jinlida combined with Tongxinluo on the injury of islet microvascular endothelial cells (MS-1) induced by high glucose and its mechanism. Methods the cell survival rate was determined by MTS method. The cells were divided into normal group, (Normal), model group, (Model), Zinlida group, (JLD), Tongxinluo group, (TXL) group and Zinrida Tongxinluo group (JLD TXL), which were treated with high glucose (50 mmol L-1) medium to establish the injury model and give drug intervention. MTS assay was used to detect the cell survival rate after 48 h and 2 weeks, and the contents of nitric oxide (NO), endothelin-1 (ET-1), superoxide dismutase (SOD) and malondialdehyde (MDA) in the supernatant at 48 h. The (ROS) level of reactive oxygen species was measured by flow cytometry. Results JLD 200mg L -1 TXL 100 mg L -1 was first determined as the administration concentration. Compared with the model group, the cell survival rate and the content of NO in the supernatant of the JLD,JLD TXL group were increased at 48 h and 2 weeks (P0.05, P0.01), while the ET-1 content in the supernatant of the JLD,JLD TXL group was decreased (P0.05, P0.01). The content of SOD in supernatant of JLD group was increased (P0.05). The content of MDA in supernatant of), JLD TXL group was decreased (P0.05). The level of intracellular ROS decreased in the administration group (P0.05, P0.01). Conclusion Jinlida combined with Tongxinluo can improve endothelial function damage of MS-1 cells induced by high glucose and play a protective role by reducing oxidative stress damage.
【作者单位】：河北以岭医药研究院;国家中医药管理局重点研究室(心脑血管络病);河北省络病重点实验室;河北医科大学附属以岭医院(国家中医药管理局中医络病学重点学科);
【基金】：国家重点基础研究发展计划(973计划)资助项目(No2012CB518606) 河北省自然科学基金资助项目(No H2013106139)
【分类号】：R96

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