丹那唑抑制大鼠线粒体呼吸链复合酶Ⅰ导致原代肝细胞坏死
发布时间：2019-02-14 10:12
【摘要】：目的研究丹那唑对大鼠原代肝细胞的损伤作用与机制。方法原代大鼠肝细胞贴壁培养4 h,加入丹那唑0,50,100,200μmol/L,4 h和24 h后采用乳酸脱氢酶法测定细胞活力并检测三磷酸腺苷(ATP)水平,同时提取线粒体,分别测5个呼吸链复合酶(complex Ⅰ~V)活性。培养液中加入20 mmol/L果糖促进糖酵解,观察果糖对丹那唑细胞毒性的影响。结果丹那唑50,100μmol/L处理4 h和24 h对细胞几乎没有杀伤作用,但可致ATP显著降低;200μmol/L处理4 h和24 h均可导致细胞坏死。果糖可对抗丹那唑4 h具杀伤作用,但对24 h杀伤作用无效。丹那唑(50~200μmol/L)显著抑制线粒体呼吸链复合酶Ⅰ活性,但不影响complexⅡ~V活性。结论丹那唑选择性抑制线粒体呼吸链复合酶Ⅰ活性而导致ATP生成减少并诱发细胞坏死。
[Abstract]:Objective to study the damage effect and mechanism of danazol on primary rat hepatocytes. Methods Primary rat hepatocytes were cultured for 4 h, then added with danazol 050100200 渭 mol/L,4 / h and 24 h later, the cell viability and adenosine triphosphate (ATP) levels were measured by lactate dehydrogenase assay, and mitochondria were extracted simultaneously. The activities of five respiratory chain complex enzymes (complex 鈪，
本文编号：2422114
[Abstract]:Objective to study the damage effect and mechanism of danazol on primary rat hepatocytes. Methods Primary rat hepatocytes were cultured for 4 h, then added with danazol 050100200 渭 mol/L,4 / h and 24 h later, the cell viability and adenosine triphosphate (ATP) levels were measured by lactate dehydrogenase assay, and mitochondria were extracted simultaneously. The activities of five respiratory chain complex enzymes (complex 鈪，
本文编号：2422114
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