Ussing chamber技术评价P-糖蛋白及有机阳离子转运体对左氧氟沙星跨胃黏膜转运的影响

发布时间：2019-03-06 14:40

【摘要】：目的评价P-糖蛋白及有机阳离子转运体对左氧氟沙星跨胃黏膜转运的影响。方法用大鼠胃黏膜与Ussing chamber技术建立胃黏膜体外模型,分为空白组及加入P-糖蛋白抑制剂(环孢素A)或有机阳离子转运体抑制剂(西咪替丁)的抑制剂组并进行双向转运实验,用高效液相色谱法对左氧氟沙星进行定量分析,计算其表观渗透系数(Papp)。结果加入环孢素A后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)由2.20变为1.06(P0.05);加入西咪替丁后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)与空白组比较,差异无统计学意义。结论 P-糖蛋白参与左氧氟沙星跨胃黏膜转运过程,而有机阳离子转运体不参与左氧氟沙星跨胃黏膜转运过程。
[Abstract]:Objective to evaluate the effects of P-glycoprotein and organic cation transporter on transgastric transport of levofloxacin. Methods the rat gastric mucosa model in vitro was established by Ussing chamber technique. They were divided into two groups: blank group, inhibitor of P-glycoprotein (cyclosporin A) or cimetidine (cimetidine), and the two-way transport experiment was carried out, and the quantitative analysis of levofloxacin was carried out by high performance liquid chromatography (HPLC). Calculation of apparent permeability coefficient (Papp). Results after addition of cyclosporine A, 50 渭 g 路mL ~ (- 1) of levofloxacin Papp / Papp (Mus) was changed from 2.20 to 1.06 (P0.05). When cimetidine was added, there was no significant difference between 50 渭 g 路mL-1 levofloxacin Papp / Papp (Mus) group and the blank group. Conclusion P-glycoprotein is involved in the transgastric transport of levofloxacin, but organic cation transporters are not involved in the transgastric transport of levofloxacin.
【作者单位】：安徽医科大学第一附属医院科研处;安徽省消化系病重点实验室;安徽医科大学第一附属医院消化内科;
【基金】：国家自然科学基金青年基金资助项目(81100269)
【分类号】：R965

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