去乙酰毛花苷和米力农对大鼠血流动力学作用的比较
发布时间：2019-03-07 16:29
【摘要】：目的研究强心苷类正性肌力代表药去乙酰毛花苷与非强心苷类正性肌力代表药米力农的心血管动力学作用特点,分析其长期使用死亡率差别的可能机制。方法 1大鼠在体压力-容积环分析:经左侧颈外静脉缓慢推注去乙酰毛花苷0.17 mg·kg~(-1)或米力农0.78 mg·kg~(-1),Miller导管记录大鼠压力-容积环变化及动脉压。2大鼠离体心脏左心室收缩压测定:实验按正常灌流液→0.01→0.1→1μmol·L~(-1)浓度梯度灌流去乙酰毛花苷,或正常灌流液→0.1→1→10μmol·L~(-1)灌流米力农。3大鼠心肌细胞钙释放测定:测定去乙酰毛花苷或米力农各10μmol·L~(-1)加药前后钙释放。结果 1在体实验表明:去乙酰毛花苷显著增加大鼠动脉压与心室收缩末期压、心输出量、搏出功、心室收缩末期压最大上升速率和心室收缩弹性;降低舒张弹性及心室收缩末期容积;延长动脉收缩压回复50%的时间;缩短主动脉瓣关闭时间(P0.05)。米力农显著降低大鼠动脉压与室收缩末期压、降低心室收缩及舒张末期容积、搏出功;增加每搏输出量、射血分数、输出量、心率、心室收缩末期压与舒张弹性;缩短心室内压及动脉收缩压回复50%的时间(P0.05)。2两者均能浓度依赖性地增加离体大鼠心脏收缩的发展力及收缩压最大上升速率,但去乙酰毛花苷减慢心率,而米力农增加心率(P0.05)。3两者均能增加大鼠心肌细胞钙释放幅值,米力农还能缩短钙回吸收时间(P0.05)。结论去乙酰毛花苷具有正性肌力作用,而对外周阻力无明显影响。米力农发挥正性肌力作用,但有明显的扩张血管作用,且明显增加心率;这些作用可能是米力农长期使用导致死亡率增加的原因。
[Abstract]:Aim to study the cardiovascular dynamic characteristics of deacetythrin, a positive inositive agent of cardiac glycosides, and milrinone, a non-cardiosides positive inotropic drug, and to analyze the possible mechanism of the difference in mortality between long-term use of amrinone. Methods 1in vivo pressure-volume loop analysis: deacetylinoside 0.17 mg kg~ (- 1) or milrinone 0.78 mg kg~ (- 1) was slowly injected into the left external jugular vein. The changes of pressure-volume ring and arterial pressure were recorded by Miller catheter. 2 the systolic pressure of left ventricle in isolated rat heart was measured by perfusion of deacetythrin at the concentration of 0. 01 to 0. 1 渭 mol 路L ~ (- 1) in normal perfusate. [WT5 "HZ] [WT5" BZ] [WT5BZ] Results 1Deacetyloside significantly increased arterial pressure, ventricular end systolic pressure, cardiac output, pulsatile work, maximal rise rate of ventricular end systolic pressure and ventricular systolic elasticity in rats. Reduce diastolic elasticity and ventricular end systolic volume; prolong arterial systolic pressure recovery time by 50%; shorten aortic valve closure time (P0.05). Milrinone significantly decreased arterial pressure and end-systolic pressure, reduced ventricular systolic and diastolic volumes, and increased stroke output, ejection fraction, output, heart rate, ventricular end-systolic pressure and diastolic elasticity, as well as ventricular systolic and diastolic volume, and increased stroke output, ejection fraction, output, heart rate, ventricular end-systolic pressure and diastolic elasticity. (2) both of them could increase the contractility of isolated rat heart in a concentration-dependent manner and the maximal increase rate of systolic blood pressure, but deacetyloside slowed down the heart rate, but deacetyloside decreased the heart rate in a concentration-dependent manner. While milrinone increased the heart rate (P0.05). 3 both of them could increase the amplitude of calcium release from rat cardiac myocytes, and milrinone could shorten the calcium absorption time (P0.05). Conclusion Deacetyloside has positive inotropic effect, but has no obvious effect on peripheral resistance. Milrinone plays a positive inotropic role, but has a significant vasodilating effect and significantly increases heart rate, which may be the cause of the increase in mortality caused by long-term use of milrinone.
【作者单位】： 南京中医药大学药学院国家科技部规范化中药药理实验室;南京军区总医院干部病房呼吸科;南京中医药大学第一附属医院(江苏省中医院)心脏内科;泰州中国医药城中医药研究院;
【基金】：2014年度江苏省高校自然科学研究重大项目(14KJA360002) 江苏省自然科学基金(BK20151355);江苏省自然科学基金(BK20131262) 泰州中国医药城第四批次高层次创新人才“113人才计划”(2016024)~~
【分类号】：R965
，
本文编号：2436266
[Abstract]:Aim to study the cardiovascular dynamic characteristics of deacetythrin, a positive inositive agent of cardiac glycosides, and milrinone, a non-cardiosides positive inotropic drug, and to analyze the possible mechanism of the difference in mortality between long-term use of amrinone. Methods 1in vivo pressure-volume loop analysis: deacetylinoside 0.17 mg kg~ (- 1) or milrinone 0.78 mg kg~ (- 1) was slowly injected into the left external jugular vein. The changes of pressure-volume ring and arterial pressure were recorded by Miller catheter. 2 the systolic pressure of left ventricle in isolated rat heart was measured by perfusion of deacetythrin at the concentration of 0. 01 to 0. 1 渭 mol 路L ~ (- 1) in normal perfusate. [WT5 "HZ] [WT5" BZ] [WT5BZ] Results 1Deacetyloside significantly increased arterial pressure, ventricular end systolic pressure, cardiac output, pulsatile work, maximal rise rate of ventricular end systolic pressure and ventricular systolic elasticity in rats. Reduce diastolic elasticity and ventricular end systolic volume; prolong arterial systolic pressure recovery time by 50%; shorten aortic valve closure time (P0.05). Milrinone significantly decreased arterial pressure and end-systolic pressure, reduced ventricular systolic and diastolic volumes, and increased stroke output, ejection fraction, output, heart rate, ventricular end-systolic pressure and diastolic elasticity, as well as ventricular systolic and diastolic volume, and increased stroke output, ejection fraction, output, heart rate, ventricular end-systolic pressure and diastolic elasticity. (2) both of them could increase the contractility of isolated rat heart in a concentration-dependent manner and the maximal increase rate of systolic blood pressure, but deacetyloside slowed down the heart rate, but deacetyloside decreased the heart rate in a concentration-dependent manner. While milrinone increased the heart rate (P0.05). 3 both of them could increase the amplitude of calcium release from rat cardiac myocytes, and milrinone could shorten the calcium absorption time (P0.05). Conclusion Deacetyloside has positive inotropic effect, but has no obvious effect on peripheral resistance. Milrinone plays a positive inotropic role, but has a significant vasodilating effect and significantly increases heart rate, which may be the cause of the increase in mortality caused by long-term use of milrinone.
【作者单位】： 南京中医药大学药学院国家科技部规范化中药药理实验室;南京军区总医院干部病房呼吸科;南京中医药大学第一附属医院(江苏省中医院)心脏内科;泰州中国医药城中医药研究院;
【基金】：2014年度江苏省高校自然科学研究重大项目(14KJA360002) 江苏省自然科学基金(BK20151355);江苏省自然科学基金(BK20131262) 泰州中国医药城第四批次高层次创新人才“113人才计划”(2016024)~~
【分类号】：R965
，
本文编号：2436266
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