两种丹酚酸注射剂在小鼠体内组织分布的研究

发布时间：2019-03-13 08:07

【摘要】：目的:注射用丹参多酚酸盐和注射用丹参多酚酸是临床常用的两种丹酚酸类注射剂,两者主要成分均为丹酚酸B,本文以丹酚酸B为检测指标,初步探究两种注射剂在小鼠体内的组织分布情况。方法:雄性ICR小鼠随机(数字表法)分为A组(注射用丹参多酚酸盐组)、B组(注射用丹参多酚酸组),剂量均为250 mg·kg~(-1)(以丹酚酸B含量计),两组每次随机(数字表法)取5只小鼠于尾静脉注射,1,5,10,15,30,60 min后摘眼球取血并颈椎脱臼处死,迅速分离心、肝、脾、肺、肾、脑组织,采用高效液相色谱(HPLC)法测定血清及各组织中丹酚酸B的含量。结果:给药1 min时,B组肝、脾、肾组织的丹酚酸B含量显著高于A组(P0.05);给药10 min时,B组小鼠心、脾、肺、脑组织中丹酚酸B的浓度显著高于A组(P0.05);给药15 min时,B组小鼠心、肝、脾、肺、肾组织中丹酚酸B的浓度均显著高于A组(P0.05);给药30 min时,B组小鼠肝、脾、肺、肾、脑组织中丹酚酸B的浓度均显著高于A组(P0.05);给药期间,B组脑组织中丹酚酸B的含量均高于A组,且5,10,30 min时有显著差异(P0.05)。结论:本实验初步研究了两种丹酚酸注射剂在小鼠体内的组织分布规律,注射用丹参多酚酸的脑药浓度高于注射用丹参多酚酸盐,可能与其制剂中所含甘露醇开放血脑屏障作用有关。
[Abstract]:Objective: Salvianolic acid for injection and Salvianolic acid for injection are two kinds of Salvianolic acid injections commonly used in clinic. The main components of Salvianolic acid B are Salvianolic acid B. in this paper, Salvianolic acid B is used as the detection index. To investigate the tissue distribution of the two injections in mice. Methods: male ICR mice were randomly divided into group A (Salvianolic acid injection group,), B group). The dosage was 250 mg kg~ (- 1) (based on Salvianolic acid B content). At random, 5 mice were injected into tail vein, 1,5,10,15,30,60 min later, the blood was extracted and the cervical vertebra was dislocated to death, and the heart, liver, spleen, lung, kidney and brain tissue were separated rapidly, and the mice in the two groups were divided into four groups: the heart, the liver, the spleen, the lung, the kidney and the brain tissue. The content of Salvianolic acid B in serum and tissues was determined by high performance liquid chromatography (HPLC). Results: the content of Salvianolic acid B in liver, spleen and kidney of group B was significantly higher than that of group A at 1 min (P0.05), and the concentration of Salvianolic acid B in heart, spleen, lung and brain tissue of group B was significantly higher than that of group A at 10 min (P0.05). At 15 min, the concentration of Salvianolic acid B in heart, liver, spleen, lung and kidney of group B was significantly higher than that of group A (P0.05). At 30 min, the concentration of Salvianolic acid B in liver, spleen, lung, kidney and brain tissue of group B was significantly higher than that of group A (P0.05). During administration, the content of Salvianolic acid B in brain tissue of group B was higher than that of group A, and there was significant difference at 5,10,30 min (P0.05). Conclusion: the distribution of Salvianolic acid and Salvianolic acid in mice was studied. The brain concentration of Salvianolic acid injection was higher than that of Salvianolic acid injection. It may be related to the opening of blood-brain barrier by mannitol.
【作者单位】：吉林大学第一医院药学部;
【分类号】：R965

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