EGCG对大鼠三硝酸苯磺酸结肠炎Caspase-1表达及其相关细胞因子分泌的影响

发布时间：2019-03-16 19:00

【摘要】：目的:观察表没食子儿茶素没食子酸酯(EGCG)对大鼠TNBS结肠炎的治疗作用,并探讨其可能的抗炎机制。方法:用TNBS/乙醇溶液灌肠建立大鼠TNBS结肠炎模型,造模成功后随机分模型组和EGCG组(浓度分别为25、50、75mg/kg),另设空白对照组。各组按相应剂量EGCG配成2ml灌胃,空白对照组和模型组给予生理盐水灌胃,连续7天。观察大鼠一般情况、进行疾病活动指数(DAI)、结肠大体形态损伤指数(CMDI)、结肠组织病理学(HI)评分;测定结肠组织匀浆丙二醛(MDA)含量、髓过氧化物酶(MPO)活性;免疫组织化学法检测结肠组织Caspase-1的表达;ELISA法检测血清中IL-1β和IL-18的水平。结果:与模型组大鼠相比,EGCG 75mg/kg浓度时,大鼠一般状况改善,DAI、CMDI和HI评分均有不同程度降低,结肠组织匀浆中MPO活性和MDA含量也有不同程度下降,免疫组化显示结肠组织中Caspase-1表达下降,血清中IL-1β和IL-18浓度也有所降低。结论:EGCG能减轻TNBS结肠炎大鼠结肠炎症,可能与EGCG抑制结肠组织中Caspase-1的表达,降低血清中IL-1β和IL-18的水平相关。
[Abstract]:Aim: to observe the therapeutic effect of epigallocatechin gallate (EGCG) on TNBS colitis in rats and to explore its anti-inflammatory mechanism. Methods: the rat model of TNBS colitis was established by enema with TNBS/ ethanol solution. The rats were randomly divided into two groups: model group (25,50,75 mg / kg) and control group (control group). Each group was fed with 2ml according to the corresponding dose of EGCG, and the blank control group and the model group were given normal saline for 7 days. The disease activity index (DAI), colonic morphological injury index (CMDI), histopathological (HI) score (HI), malondialdehyde (MDA) content and myeloperoxidase (MPO) activity in colon tissue homogenate were observed, and the activity of myeloperoxidase (MPO) in colon tissue was measured. The expression of Caspase-1 in colon tissue was detected by immunohistochemical method, and the levels of IL-1 尾 and IL-18 in serum were detected by ELISA method. Results: compared with the model group, the general condition of rats was improved, the scores of DAI,CMDI and HI were decreased, and the activity of MPO and the content of MDA in colon tissue homogenate were also decreased in different degrees when the concentration of EGCG 75mg/kg was higher than that of the model group. Immunohistochemistry showed that the expression of Caspase-1 and the concentration of IL-1 尾 and IL-18 in the colon tissue were lower than those in the control group. Conclusion: EGCG can alleviate colon inflammation in rats with TNBS colitis, which may be related to EGCG inhibiting the expression of Caspase-1 in colon tissue and decreasing the levels of IL-1 尾 and IL-18 in serum.
【作者单位】：福建医科大学附属第一医院消化内科;福建医科大学消化系病研究室;
【基金】：福建医科大学学科带头人专项基金(编号:JXK200701) 福建省自然科学基金项目(编号:2015J01391)
【分类号】：R965

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