微透析-高效液相色谱法联用研究吴茱萸提取物的经皮吸收药动学特性

发布时间：2019-03-18 17:29

【摘要】：目的:利用在体皮肤微透析技术研究吴茱萸提取物经皮吸收的特性。方法:以裸鼠为研究对象,建立在体经皮微透析采样技术,以吴茱萸碱和吴茱萸次碱为指标成分,采用高效液相色谱测定吴茱萸提取物经皮给药后透析液中的药物浓度,通过相对损失率的校正,计算皮肤药物浓度,并利用Kinetica 5.0软件对皮肤药物浓度和时间进行非房室模型拟合,计算相关统计参数。结果:吴茱萸提取物中吴茱萸碱的达峰时间为(150±15.3)min,半衰期(t1/2)(263.7±41.6)min;吴茱萸次碱的达峰时间为(90±2.1)min,半衰期(t1/2)(194±17.3)min;二者均能较快地达到峰值,并在较长的时间内保持稳定释放,使皮下组织中的药物浓度保持在一个相对恒定的水平。结论:本研究中所建立的微透析方法可用于吴茱萸提取物的皮肤药动学研究,吴茱萸碱和吴茱萸次碱可透过皮肤吸收而发挥临床药效。
[Abstract]:Objective: to study the characteristics of percutaneous absorption of Evodia officinalis extract by in vivo skin microdialysis. Methods: the in vivo percutaneous microdialysis sampling technique was established in nude mice. The concentration of Evodia officinalis extract in dialysate solution was determined by high performance liquid chromatography (HPLC) using evodipine and Evodia officinalis as the index components, and the drug concentration was determined by high performance liquid chromatography (HPLC) after transdermal administration of Evodia officinalis extract. The skin drug concentration was calculated by the correction of relative loss rate, and the non-atrioventricular model was used to fit the skin drug concentration and time with Kinetica 5.0 software, and the relevant statistical parameters were calculated. Results: the peak time of evodiamine was (150 卤15.3) min, (263.7 卤41.6) min;. The peak time of evodipine was (90 卤2.1) min,. (194 卤17.3) min;. Both of them could reach the peak value quickly and keep stable release for a long time so that the drug concentration in subcutaneous tissue was kept at a relatively constant level. Conclusion: the microdialysis method established in this study can be used to study the skin pharmacokinetics of Evodia officinalis extract. Evodipine and evodipine can be absorbed through the skin to exert the clinical efficacy.
【作者单位】：广州中医药大学第一附属医院;广州中医药大学;
【基金】：广州中医药大学科研创新基金(编号:11CX027)
【分类号】：R96

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