典型滤光剂纳米二氧化钛与2-羟基-4-甲氧基二苯甲酮联合染毒及其在鲫鱼体内蓄积和对肝抗氧化酶活力的影响

发布时间：2019-03-30 16:09

【摘要】：目的探讨典型滤光剂纳米二氧化钛与2-羟基-4-甲氧基二苯甲酮(BP3)联合染毒条件下,两者在鲫鱼不同组织的蓄积及对鲫鱼肝抗氧化酶活力的影响。方法将210尾健康12月龄雌性鲫鱼随机分成7组,分别为空白对照(曝气自来水)组、溶剂对照(0.01%甲醇)组和4、20μg/L BP3染毒组及100μg/L纳米二氧化钛染毒组以及4μg/L BP3+100μg/L纳米二氧化钛染毒组、20μg/L BP3+100μg/L纳米二氧化钛染毒组,每组30尾。采用半静态方式进行染毒,每天更换50%染毒溶液。分别在染毒7、14 d时,测定鲫鱼脑、肝脏、鳃、胆汁、肠、肌肉组织中纳米二氧化钛和BP3的含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的活力。结果空白对照组、溶剂对照组和纳米二氧化钛单独染毒组鲫鱼体内均未检出BP3。BP3单独染毒组中,BP3在鲫鱼肝和肠中的含量最高,分别为49.5~758.81 ng/g和40.12~890.32 ng/g;肌肉和胆汁的BP3含量最低,为4.38~24.72 ng/g。与相同浓度BP3单独染毒组比较,各浓度BP3+纳米二氧化钛联合染毒第7、14天时鲫鱼各组织中BP3的含量均较高,除肌肉和胆汁外,差异均有统计学意义(P0.05);且随着BP3和纳米二氧化钛染毒浓度的升高及染毒时间的延长,鲫鱼各组织中BP3的含量均呈上升趋势。空白对照组、溶剂对照组和BP3单独染毒组鲫鱼体内均未检出纳米二氧化钛。与溶剂对照组比较,第7、14天时20μg/L BP3染毒组和20μg/L BP3+100μg/L纳米二氧化钛染毒组鲫鱼肝脏SOD、CAT和GPx活力升高,差异均有统计学意义(P0.05);而空白对照组、4μg/L BP3染毒组、100μg/L纳米二氧化钛染毒组、4μg/L BP3+100μg/L纳米二氧化钛染毒组鲫鱼肝脏SOD、CAT和GPx活力均无明显变化。与相同浓度BP3单独染毒组比较,各浓度BP3+纳米二氧化钛染毒组鲫鱼肝脏SOD、CAT和GPx的活力升高,差异均有统计学意义(P0.05)。结论纳米二氧化钛的存在促进了BP3在鱼体鳃、肝、肠和脑中的蓄积,20μg/L的BP3会提高鲫鱼肝抗氧化酶活力,且共存的纳米二氧化钛促进鱼体肝脏抗氧化酶活力的升高。
[Abstract]:Aim to investigate the accumulation of nano-titanium dioxide and 2-hydroxy-4-methoxybenzophenone (BP3) in different tissues of Carassius auratus and its effect on the activity of antioxidant enzymes in the liver of crucian carp (Carassius auratus) under the combined exposure of nano-titanium dioxide and 2-hydroxy-4-methoxybenzophenone (BP3). Methods 210 healthy 12-month-old female crucian carp were randomly divided into 7 groups: blank control group (aerated tap water) group. Solvent control (0.01% methanol) group, 4,20 渭 g / L BP3 group, 100 渭 g / L nano-titanium dioxide group, 4 渭 g / L BP3 100 渭 g / L nano-titanium dioxide group, 20 渭 g / L BP3 100 渭 g / L nano-titanium dioxide group, 30 rats in each group. Semi-static method was used for poisoning, and 50% of the solution was replaced every day. The contents of nano-TIO _ 2 and BP3 and superoxide dismutase (SOD), in brain, liver, Gill, bile, intestine and muscle of Carassius auratus were determined at 7 and 14 days, respectively. Activities of catalase (CAT) and glutathione peroxidase (GPx). Results No BP3.BP3 was detected in the blank control group, solvent control group and nano titanium dioxide alone group, and the content of BP3 in liver and intestine of crucian carp was the highest. 49.5 / 758.81 ng/g and 40.12 / 890.32 ng/g;, respectively. The lowest BP3 content was 4.38 to 24.72 ng/g. in muscle and bile. Compared with the same concentration of BP3 alone, the content of BP3 in all tissues of Carassius auratus was higher at the 7th and 14th day after exposure to BP3 nano-titanium dioxide (P0.05). Except for muscle and bile, the difference was statistically significant (P0.05). With the increase of the concentration of BP3 and nano-TIO _ 2 and the prolongation of exposure time, the content of BP3 in all tissues of crucian carp showed an increasing trend. No nano-TIO _ 2 was detected in crucian carp (Carassius auratus L.) exposed to BP3 alone, solvent control group and blank control group. Compared with the solvent control group, the activities of SOD,CAT and GPx in liver of Carassius auratus exposed to 20 渭 g / L BP3 and 20 渭 g / L BP3 100 渭 g / L nano-TIO _ 2 increased significantly on the 7th and 14th day (P0.05). There were no significant changes in SOD,CAT and GPx activities in liver of Carassius auratus in blank control group, 4 渭 g / L BP3 group, 100 渭 g / L nano-TIO _ 2 group and 4 渭 g / L BP3 100 渭 g / L nano-TIO _ 2 group. Compared with the same concentration of BP3 alone, the activities of SOD,CAT and GPx in the liver of crucian carp exposed to nano-TIO _ 2 with different concentrations of BP3 were significantly higher than those of the other groups (P0.05). Conclusion the presence of nano-TIO _ 2 promotes the accumulation of BP3 in the Gill, liver, intestine and brain of fish. 20 渭 g / L BP3 can increase the activity of antioxidant enzymes in the liver of Carassius auratus, and the coexistence of nano-TIO _ 2 promotes the increase of antioxidant enzyme activity in fish liver.
【作者单位】：河海大学环境学院浅水湖泊综合治理与资源开发教育部重点实验室;
【基金】：国家自然科学基金(51509071) 江苏省自然科学基金(BK20150801) 河海大学中央高校业务费(2014B12514)
【分类号】：R994.6

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