鞘内注射P2X7受体拮抗剂BBG对大鼠行为学和脊髓PLA2表达的影响

发布时间：2019-03-30 22:09

【摘要】：目的:探讨鞘内注射P2X7受体拮抗剂考马斯亮蓝(BBG)在福尔马林模型大鼠中的镇痛作用及对脊髓磷脂酶A2(PLA2)表达的影响。方法:雄性Wistar大鼠鞘内置管成功后鞘内注射P2X7受体拮抗剂考马斯亮蓝(BBG)后所有的大鼠左侧后肢爪掌背侧皮下注射福尔马林(5%,50μl),观察大鼠在第一相(0~10 min)和第二相(10~60 min)的疼痛行为学比,变化(自发缩足次数)。免疫组织化学方法检测脊髓背角PLA2蛋白的表达与变化。结果:各组间大鼠第一相相比自发缩足次数没有明显的差异;第二相与对照组相比鞘内1μg、5μg及腹腔10μg BBG注射组相比,鞘内10μg BBG注射组大鼠的疼痛行为学明显减少(自发缩足次数,P0.05)。与此同时,鞘内10μg BBG注射组大鼠脊髓背角浅层内PLA_2免疫阳性产物的表达与其它组比较明显减少(P0.05)。结论:鞘内注射10μg BBG可减轻福尔马林诱导的炎性痛觉过敏,可能是通过抑制脊髓背角浅层PLA2的表达而发挥作用。
[Abstract]:Aim: to investigate the analgesic effect of intrathecal injection of P2X7 receptor antagonist Coomassie brilliant blue (BBG) in formalin model rats and the effect of intrathecal injection on the expression of phospholipase A 2 (PLA2) in spinal cord. Methods: after intrathecal injection of P2X7 receptor antagonist Coomassie brilliant blue (BBG), formalin (5%, 50 渭 l) was subcutaneously injected into the dorsal claw metacarpal of the left hind limb of male Wistar rats. The pain behavior ratios and changes in the first phase (0? 10 min) and the second phase (10? 60 min) were observed. Immunohistochemical method was used to detect the expression and change of PLA2 protein in spinal dorsal horn. Results: there was no significant difference in the frequency of spontaneous foot contraction between the first phase and the first phase of the rats. In the second phase, compared with the control group, 1 渭 g, 5 渭 g intrathecal and 10 渭 g BBG injected intrathecally, the pain behavior of the rats in the intrathecal 10 渭 g BBG injection group was significantly decreased (P 0.05). At the same time, the expression of PLA_2 immunopositive products in the superficial layer of spinal dorsal horn in the intrathecal 10 渭 g BBG injection group was significantly lower than that in the other groups (P0.05). Conclusion: intrathecal injection of 10 渭 g BBG can reduce formalin-induced inflammatory hyperalgesia, which may be due to the inhibition of the expression of PLA2 in the spinal dorsal horn.
【作者单位】：山西大同大学医学院生理学教研室;
【基金】：山西大同大学博士科研启动经费
【分类号】：R965

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