核磁共振波谱法测定盐酸卡替洛尔等9种洛尔类药物对照品的绝对含量

发布时间：2019-04-19 06:18

【摘要】：目的采用核磁共振波谱法测定盐酸卡替洛尔、盐酸塞利洛尔、盐酸贝凡洛尔、盐酸艾司洛尔、盐酸索他洛尔、阿替洛尔、盐酸普萘洛尔、盐酸拉贝洛尔和酒石酸美托洛尔共9种洛尔类药物对照品的绝对含量。方法以三聚甲醛、对苯二酚和苯甲酸分别为9种洛尔类药物对照品的内标,与待测物混合并同时测定,通过比较洛尔类药物定量峰与内标物定量峰峰面积的比值确定待测物的含量。采用核磁定量的方法,波谱条件以zg30脉冲序列获取"H-NMR谱,5 mm BBO(宽带观测)探头,延迟时间2 s,采样次数32次,谱宽9 615.385 Hz。结果核磁共振法测定结果与各对照品标示值误差约为1%。结论该方法准确、专属、快速,适用于标定药物基准物质的绝对含量。
[Abstract]:Objective to establish a nuclear magnetic resonance spectroscopy (NMR) method for the determination of carteprolol hydrochloride, serilol hydrochloride, befanolol hydrochloride, esmolol hydrochloride, sotalol hydrochloride, atenolol hydrochloride, propranolol hydrochloride, and propranolol hydrochloride. Absolute contents of 9 kinds of prolol reference substances in Rabelolol Hydrochloride and Metoprolol tartrate. Methods using triformaldehyde, hydroquinone and benzoic acid as the internal standard of 9 kinds of prolol drugs, mixed with the tested substance and determined at the same time. By comparing the ratio of the quantitative peak of prolol to the area of the quantitative peak of the internal standard, the content of the substance to be determined was determined. The "H-NMR spectrum, 5 mm BBO (broadband observation) probe was obtained by zg30 pulse sequence using NMR quantitative method. The delay time was 2 s, the sampling times were 32 times and the spectral width was 9 615.385 Hz.. Results the error between the result of NMR and the standard value of each reference sample was about 1%. Conclusion the method is accurate, specific, rapid and suitable for the calibration of the absolute content of drug reference substances.
【作者单位】：中国医科大学辽阳中心医院药学部;辽宁药联制药有限公司;沈阳药科大学药学院;
【分类号】：R927;O657.2

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