右美托咪定对小鼠视网膜缺血再灌注损伤的保护机制

发布时间：2019-04-26 12:52

【摘要】：目的研究右美托咪定对小鼠视网膜缺血再灌注损伤的保护作用机制。方法选取8周龄C57BL/6雄性小鼠48只,按照体重随机分为3组:假手术组、模型组和实验组,每组16只。制备小鼠视网膜缺血再灌注损伤(RIRI)模型。在缺血前15 min和再灌注前5 min,实验组小鼠分别腹腔注射右美托咪定25μg·kg~(-1),假手术组和模型组腹腔注射同等剂量的0.9%Na Cl。在模型建立成功后,于再灌注24 h处死并取材。取小鼠视网膜组织匀浆液,用四唑盐试剂(WST-1)法检测超氧化物歧化酶(SOD),用TBA法检测丙二醛(MDA),用比色法测定谷胱甘肽过氧化物酶(GSH-PX),用酶联免疫吸附法检测肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、1-甲基环丙烯(1-MCP)及IL-10水平。结果与假手术组比较,模型组小鼠视网膜组织中SOD为(45.47±8.16)U·mg~(-1)、GSH-PX为(264.64±27.31)U·mg~(-1),均明显降低;而MDA为(1.56±0.41)nmol·mg~(-1)、TNF-α为(2.67±0.23)ng·mL~(-1)、IL-6为(2.84±0.34)ng·mL~(-1)、MCP-1为(0.68±0.06)ng·mL~(-1)和IL-10为(0.21±0.02)ng·mL~(-1),均明显升高,差异有统计学意义(均P0.05)。与模型组比较,实验组小鼠视网膜组织中SOD为(71.05±9.34)U·mg~(-1)、GSH-PX为(382.20±31.56)U·mg~(-1)、IL-10为(0.44±0.07)ng·mL~(-1),均明显降低;而MDA为(1.02±0.23)nmol·mg~(-1)、TNF-α为(1.53±0.20)ng·mL~(-1)、IL-6为(1.64±0.07)ng·mL~(-1)、MCP-1为(0.41±0.07)ng·mL~(-1),均明显降低,差异有统计学意义(P0.05)。结论右美托咪定可以改善视网膜缺血再灌注损伤,其机制与抑制氧自由基所致的脂质过氧化损伤和抑制炎症因子的分泌有关。
[Abstract]:Objective to study the protective effect of right metametrimidine on retinal ischemia reperfusion injury in mice. Methods 48 8-week-old male C57BL/6 mice were randomly divided into three groups according to their weight: sham-operated group, model group and experimental group, with 16 mice in each group. The (RIRI) model of retinal ischemia reperfusion injury in mice was established. At 15 min before ischemia and 5 min, before reperfusion, the mice in the experimental group were intraperitoneally injected with 25 渭 g kg~ (- 1) of right metomidine, and the same dose of 0.9%Na Cl. was injected intraperitoneally in the sham-operated group and the model group. After the successful establishment of the model, the rats were killed at 24 h after reperfusion and the samples were obtained. The contents of superoxide dismutase (SOD), malondialdehyde (MDA),) and glutathione peroxidase (GSH-PX) in retinal homogenate of mice were determined by WST-1 method and TBA method, and glutathione peroxidase (GSH-PX) was determined by colorimetric method. The levels of tumor necrosis factor-伪 (TNF- 伪), interleukin-6 (IL-6), 1-methylcyclopropene (1-MCP) and IL-10 were measured by enzyme-linked immunosorbent assay (Elisa). Results compared with the sham-operated group, the SOD and GSH-PX in the retinal tissue of the model group were (45.47 卤8.16) U mg~ (- 1) and (264.64 卤27.31) U mg~ (- 1). While MDA, TNF- 伪 and IL-6 were (1.56 卤0.41) nmol mg~ (- 1), (2.67 卤0.23) ng 路mL ~ (- 1) and (2.84 卤0.34) ng 路mL ~ (- 1), respectively. MCP-1 was (0.68 卤0.06) ng 路mL ~ (- 1) and IL-10 was (0.21 卤0.02) ng 路mL ~ (- 1), the difference was statistically significant (P 0.05). Compared with the model group, the SOD, GSH-PX and IL-10 were (71.05 卤9.34) U mg~ (- 1), (382.20 卤31.56) U mg~ (- 1) and (0.44 卤0.07) ng 路mL ~ (- 1) in the experimental group. All of them decreased significantly. While MDA, TNF- 伪, IL-6 and MCP-1 were (1.02 卤0.23) nmol mg~ (- 1), (1.53 卤0.20) ng 路mL ~ (- 1), (1.64 卤0.07) ng 路mL ~ (- 1) and (0.41 卤0.07) ng 路mL ~ (- 1), respectively. The difference was statistically significant (P0.05). Conclusion right metametrimidine can improve retinal ischemia-reperfusion injury by inhibiting lipid peroxidation injury induced by oxygen free radicals and inhibiting the secretion of inflammatory factors. [WT5 "HZ] conclusion [WT5" BZ] [WT5 "BZ]
【作者单位】：河南大学淮河医院麻醉科;锦州医科大学研究生院;
【基金】：河南省科技攻关基金资助项目(2017-172102410009) 开封市社会发展科技攻关计划基金资助项目(2016-1603065)
【分类号】：R965

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