受体成分蛋白在降钙素基因相关肽和血管紧张素II对血管平滑肌细胞血管过氧化物酶1表达调控中的作用

发布时间：2019-05-08 18:19

【摘要】：血管紧张素II(angiotensin II,Ang II)和降钙素基因相关肽(calcitonin gene-related peptide,CGRP)在血管的损伤和保护中起重要作用。为了探讨CGRP受体成分蛋白(receptor component protein,RCP)在CGRP和Ang II对血管平滑肌细胞(vascular smooth muscle cell,VSMC)血管过氧化物酶1(vascular peroxidase-1,VPO1)表达调控中的作用及机制,本研究采用体外培养鼠源性A10血管平滑肌细胞株(A10VSMC),给予CGRP或/和Ang II刺激,并用小干扰RNA(si RNA)干扰细胞RCP基因的表达,Western Blot检测RCP以及VPO1蛋白表达水平;RT-PCR检测RCP及VPO1 m RNA的表达水平。结果显示,在静止期野生型A10VSMC,CGRP和Ang II能分别上调RCP和VPO1蛋白和m RNA表达(均P0.05),但CGRP预孵育细胞后,Ang II诱导的RCP和VPO1蛋白表达降低(均P0.05);与野生型组比较,VPO1在所有RCP基因干扰组的表达均显著降低(均P0.01)。同时,在RCP基因干扰条件下,和对照组相比,CGRP处理组VPO1蛋白的表达显著增加,而Ang II组没有明显变化;和Ang II组相比,CGRP与Ang II联合作用显著增加VPO1蛋白表达,但这种作用能被抗氧化酶Catalase所抑制(P0.05)。以上结果提示,RCP可能参与CGRP或Ang II诱导的VPO1蛋白表达;RCP可能在介导CGRP和Ang II受体共同调控VPO1表达的信号转导整合中起一定作用。
[Abstract]:Angiotensin II (angiotensin II,Ang II) and calcitonin gene-related peptide (calcitonin gene-related peptide,CGRP) play an important role in vascular injury and protection. To investigate the role and mechanism of CGRP receptor protein (receptor component protein,RCP in regulating the expression of vascular peroxidase 1 (vascular peroxidase-1,VPO1) in vascular smooth muscle cells (vascular smooth muscle cell,VSMC) induced by CGRP and Ang II. In this study, mouse A10 vascular smooth muscle cell line (A10VSMC) was cultured in vitro and stimulated by CGRP or / and Ang II. The expression of RCP gene in small interfering RNA (si RNA) interfering cells was detected by, Western Blot. The expression of RCP and VPO1 protein was detected. The expression levels of RCP and VPO1 m RNA were detected by RT-PCR. The results showed that RCP and Ang II could up-regulate the expression of RCP and VPO1 protein and m-RNA respectively (P 0.05), but the expression of RCP and VPO1 protein induced by, Ang II decreased after preincubation with CGRP (P 0.05). Compared with the wild type group, the expression of VPO1 in all RCP gene interference groups was significantly decreased (all P 0.01). At the same time, under the condition of RCP gene interference, the expression of VPO1 protein in CGRP treated group was significantly higher than that in control group, but there was no significant change in Ang II group. Compared with Ang II group, the combination of CGRP and Ang II significantly increased the expression of VPO1 protein, but this effect was inhibited by antioxidase Catalase (P0.05). These results suggest that RCP may be involved in the expression of VPO1 protein induced by CGRP or Ang II, and RCP may play a role in the signal transduction integration mediated by CGRP and Ang II receptors that co-regulate the expression of VPO1.
【作者单位】：南华大学药物药理研究所湖南省分子靶标新药研究协同创新中心;
【基金】：supported by the National Natural Science Foundation of China(No.81173060) Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study,China(No.2014-405)
【分类号】：R967

【相似文献】