法尼醇X受体基因缺失对雌性小鼠神经行为及神经传递系统的影响

发布时间：2019-05-15 22:26

【摘要】：目的探讨法尼醇X受体(farnesoid X receptor,FXR)作为一种核激素受体对情感、社交或记忆等神经行为的调节作用。方法通过一系列行为学测试,包括高架十字迷宫,强迫游泳、社交活动和避暗,以及LC-MS/MS法检测大脑不同区域的神经递质,评估FXR基因敲除(knockout,KO)对♀小鼠中枢神经系统功能的影响。结果 FXR KO导致小鼠在强迫游泳中不动时间延长(P0.01),进入高架十字迷宫开放臂次数增加(P0.01),逗留时间延长,同时社交活动增强(P0.01),但对避暗行为中的潜伏期和错误次数无明显影响。FXR KO亦导致小鼠海马γ-氨基丁酸(GABA,P0.05)、谷氨酸(Glu,P0.05)、去甲肾上腺素(NE,P0.01)以及GABA/Glu(P0.05)明显降低,但对前额叶相关神经递质无明显影响。结论 FXR可能参与调控中枢神经系统中神经递质稳态平衡,进而影响动物的情感和社交行为。
[Abstract]:Objective to investigate the regulatory effect of farnitol X receptor (farnesoid X receptor,FXR as a nuclear hormone receptor on emotional, social or memory neurobehavior. Methods A series of behavioral tests, including elevated cross maze, forced swimming, social activity and dark avoidance, and LC-MS/MS were used to detect neurotransmitters in different parts of the brain to evaluate FXR gene knockout (knockout,). Effect of KO) on central nervous system function in mice. Results FXR KO prolonged the immobility time of mice during forced swimming (P01), increased the number of open arms entering the elevated cross maze (P01), prolonged the stay time and increased the social activity (P01). However, it had no significant effect on the latency and number of errors in dark avoidance behavior. FXR KO also led to GABA,P0.05, Glu,P0.05 and NE, in hippocampus of mice. P 0.01) and GABA/Glu (P 0.05) were significantly decreased, but there was no significant effect on prefrontal lobe related neurotransmitters. Conclusion FXR may be involved in the regulation of neurotransmitter homeostasis in the central nervous system, which may affect the emotional and social behavior of animals.
【作者单位】：上海中医药大学中药研究所暨上海市复方中药重点实验室;
【基金】：教育部高等学校博士学科点专项科研基金优先发展领域项目(No 20123107130002) 上海高校特聘教授(东方学者)岗位计划资助(2013-59) 上海市教委科研创新重点项目(No 13ZZ099)
【分类号】：R965

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