慢性皮质酮注射对小鼠抑郁样行为及脑糖原水平的影响

发布时间：2019-05-22 04:15

【摘要】：目的:探讨慢性皮质酮注射对小鼠抑郁样行为以及脑糖原水平的影响。方法:将40只雄性C57BL/6N小鼠随机分为正常对照组与模型组,模型组小鼠连续4周给予皮质酮皮下注射,构建慢性应激抑郁障碍小鼠模型。采用强迫游泳实验和旷场实验验证慢性应激模型的建立;放免法测定小鼠血清中皮质酮(CORT)水平;采用蛋白免疫印迹法检测海马突触素(SYP)和脑源性神经营养因子(BDNF)的蛋白表达水平;采用荧光间接测定法检测海马组织的糖原以及糖原合酶和糖原磷酸化酶的水平。结果:与正常对照组相比,模型组强迫游泳静止不动时间延长(P0.01)、自主活动能力降低(P0.01),表明慢性皮质酮注射诱导小鼠产生抑郁样行为。抑郁小鼠的皮质酮明显升高(P0.01)。皮质酮注射降低海马SYP和BDNF的蛋白表达(P0.01),同时海马组织糖原含量减少(P0.05),糖原合酶的活性降低(P0.05),而糖原磷酸化酶活性增加(P0.05)。结论:慢性皮质酮注射引起的海马神经元损伤和诱导小鼠抑郁样行为,可能与皮质酮降低海马糖原水平有关。
[Abstract]:Objective: to investigate the effect of chronic corticosterone injection on depression-like behavior and brain glycogen level in mice. Methods: 40 male C57BL/6N mice were randomly divided into normal control group and model group. The mice in the model group were given corticosterone subcutaneous injection for 4 weeks to establish the model of chronic stress depression disorder. The establishment of chronic stress model was verified by forced swimming test and open field test, and the level of corticosterone (CORT) in serum of mice was measured by radioimmunoassay. The protein expression of synaptophysin (SYP) and brain-derived neurotrophic factor (BDNF) in hippocampus was detected by Western imprinting, and the levels of glycogen, glycogen enzyme and glycogen phosphatase in hippocampal tissue were detected by fluorescence indirect assay. Results: compared with the normal control group, the forced swimming rest time in the model group was prolonged (P01), and the ability of autonomous activity was decreased (P01), which indicated that chronic corticosterone injection induced depression in mice. Corticosterone in depressed mice was significantly increased (P 0.01). Corticosterone injection decreased the protein expression of SYP and BDNF in hippocampus (P01), while the glycogen content in hippocampal tissue decreased (P05), the activity of glycogen enzyme decreased (P05), while the activity of glycogen phosphatase increased (P05). Conclusion: the injury of hippocampal neurons induced by chronic corticosterone injection and the inhibitory behavior induced by corticosterone may be related to the decrease of glycogen level in hippocampus.
【作者单位】：广州医科大学病理教研室;南京中医药大学病理教研室;
【基金】：国家自然科学基金资助项目(No.81303246)
【分类号】：R965

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