卡维地洛、厄贝沙坦与马来酸噻吗洛尔的荷移反应研究

发布时间：2019-05-31 15:48

【摘要】：目的:建立快速定量分析卡维地洛、厄贝沙坦及马来酸噻吗洛尔制剂及血清中含量的荷移-紫外分光光度法。方法:以紫外分光光度法为手段,研究卡维地洛、厄贝沙坦和马来酸噻吗洛尔与适宜的电子受体试剂之间的电荷转移反应,优化并确立最佳反应条件。结果:(1)卡维地洛与茜素电荷转移反应最佳反应条件是:在室温下,放置30min,形成1:1型稳定的红褐色荷移络合物,最大吸收峰红移至542nm,卡维地洛的浓度在5-180mg/L范围内线性良好,相关系数是r=0.9999。用确立的方法测定胶囊、片剂中卡维地洛含量回收率在99.4-101.0%之间,血清中卡维地洛回收率在101.13-101.35%。(2)厄贝沙坦与氯冉酸电荷转移反应最佳反应条件:在室温下即刻反应,生成了1:1型在稳定的红色荷移络合物,最大吸收峰在520nm,荷移络合物的线性范围是10-800mg/L。药物制剂的回收率在100.3-101.6%之间,血清中药物回收率在99.7-100.05%。(3)马来酸噻吗洛尔与TCNQ电荷转移反应:需要在50℃下反应60min,生成的络合物最大吸收峰在842nm,药物浓度在1-25mg/L范围内符合比尔定律。用拟定方法测定片剂、滴眼剂中噻吗洛尔含量,回收率在样回收率在100.4-102.2%,RSD为1.10-3.54%,血清中马来酸噻吗洛尔回收率测定结果是100.9-101.5%。结论:三种基于电荷转移反应的分光光度法,简便、快速、准确,对药物含量测定结果令人满意;对于质量控制实验室常规应用具有实用价值。
[Abstract]:Objective: To establish a rapid and quantitative analysis of the content of carvedilol, irbesartan and the metoprolol maleate and the determination of the content of the serum. Methods: The charge transfer reaction between carvedilol, irbesartan and dimaleate and appropriate electron acceptor reagent was studied by means of ultraviolet spectrophotometry, and the optimum reaction conditions were established. Results: (1) The best reaction conditions for the charge transfer reaction of carvedilol and fluorescein were as follows: at room temperature, the optimum reaction conditions were as follows: at room temperature for 30 min, a 1:1 type of stable red-brown charge-shift complex was formed, the maximum absorption peak was shifted to 542 nm, and the concentration of carvedilol was well within the range of 5-180 mg/ L, and the correlation coefficient was r = 0.9999. The content of carvedilol in the capsule was 99.4-101.0%, and the recovery of carvedilol in the serum was 101.13-101.35%. (2) The best reaction conditions for the charge transfer reaction of irbesartan and chlordecanoic acid are as follows: the reaction is carried out at room temperature, and a 1:1 type in the stable red charge-shift complex is generated, the maximum absorption peak is 520 nm, and the linear range of the charge-shift complex is 10-800mg/ L. The recovery rate of the pharmaceutical preparation is between 100.3 and 101.6%, and the recovery rate of the drug in the serum is 99.7-100.05%. (3) The charge transfer reaction between nolol maleate and TCNQ: it is necessary to react for 60 min at 50 & deg; C, the maximum absorption peak of the generated complex is 842 nm, and the drug concentration is in the range of 1-25 mg/ L to meet the Beer's law. The recovery was 100.4-102.2%, RSD was 1.10-3.54%, and the recovery was 100.9-101.5%. Conclusion: The three kinds of spectrophotometric method, which is based on the charge transfer reaction, are simple, rapid and accurate, and the result of the determination of the drug content is satisfactory; and it is of practical value to the routine application of the quality control laboratory.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R917

【参考文献】