左氧氟沙星在肺炎克雷伯菌肺部感染小鼠体内的药动学
发布时间：2019-06-06 00:28
【摘要】：目的:建立肺炎克雷伯菌(Klebsiella pneumoniae,Kp)肺部感染小鼠模型,采用高效液相色谱(HPLC)法测定不同剂量左氧氟沙星(levofloxacin,LVF)在其体内的药动学参数。方法:血浆样品经乙腈沉淀蛋白后,采用Diamosail-C18色谱柱分离,流动相为乙腈(0.05 mol·L~(-1))-磷酸二氢钾(17∶83),磷酸二氢钾盐溶液以磷酸调整pH为3.0左右,流速为1 mL·min~(-1);检测波长为295 nm。结果:LVF在0.3~12 mg·L~(-1)内线性关系良好;方法的日间和日内精密度均小于7.2%,稳定性良好。Kp肺部感染小鼠口服LVF15,30,60和90 mg·kg~(-1)后,LVF的PK参数tmax、t1/2、CL/F和Vd/F均无显著性差异(P0.05)。结论:所建立的HPLC分析方法准确、灵敏、专属性强、重复性高,适用于肺炎克雷伯菌肺部感染小鼠体内的左氧氟沙星药动学研究。
[Abstract]:Aim: to establish a mouse model of pulmonary infection with Klebsiella pneumoniae (Klebsiella pneumoniae,Kp). The pharmacokinetics parameters of levofloxacin (levofloxacin,LVF) in vivo were determined by high performance liquid chromatography (HPLC). Methods: the plasma sample was precipitated by acetonitrile and separated by Diamosail-C18 column. The mobile phase was acetonitrile (0.05 mol 路L ~ (- 1)-potassium dihydrogen phosphate (17:83). The pH of potassium dihydrogen phosphate solution was adjusted to about 3.0 with phosphate, and the flow rate was 1 mL 路min ~ (- 1). Detection wavelength is 295 nm. Results: the linear range of LVF was 0.3 鈮，
本文编号：2493931
[Abstract]:Aim: to establish a mouse model of pulmonary infection with Klebsiella pneumoniae (Klebsiella pneumoniae,Kp). The pharmacokinetics parameters of levofloxacin (levofloxacin,LVF) in vivo were determined by high performance liquid chromatography (HPLC). Methods: the plasma sample was precipitated by acetonitrile and separated by Diamosail-C18 column. The mobile phase was acetonitrile (0.05 mol 路L ~ (- 1)-potassium dihydrogen phosphate (17:83). The pH of potassium dihydrogen phosphate solution was adjusted to about 3.0 with phosphate, and the flow rate was 1 mL 路min ~ (- 1). Detection wavelength is 295 nm. Results: the linear range of LVF was 0.3 鈮，
本文编号：2493931
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