HMGB1抗体对刀豆蛋白A引起小鼠肝损伤的保护作用

发布时间：2019-06-06 19:26

【摘要】：目的研究高迁移率族蛋白1(HMGB1)抗体对刀豆蛋白A(ConA)引起小鼠肝损伤的保护作用。方法将健康无污染雄性Balb/c小鼠分成空白对照组(注射生理盐水)、模型组(注射ConA)和实验组(注射ConA+HMGB1抗体),3组小鼠注射药物6 h后摘眼球取血,所获血液离心后取血清检测谷丙转氨酶(ALT)和HMGB1,留取肝脏组织进行HE染色、Tunel、免疫荧光等检测。结果实验组小鼠肝组织病理炎症反应轻于模型组。小鼠血清中ALT、HMGB1:空白对照组分别为(52.00±8.34)U/L、(7.54±0.53)ng/mL,模型组分别为(5 551.50±1 445.74)U/L、(18.06±1.65)ng/mL,实验组分别为(1 977.40±654.89)U/L、(10.77±0.71)ng/mL;肝组织中HMGB1 mRNA、HMGB1表达量(相对值):空白对照组分别为1.886±0.253、0.086±0.028,模型组分别为4.718±0.341、0.268±0.043,实验组分别为3.005±0.331、0.116±0.008;实验组小鼠血清中ALT、HMGB1,以及肝组织中HMGB1 mRNA、HMGB1表达量均低于模型组(均P0.001)。肝组织细胞凋亡、肝细胞内HMGB1迁移(标准化):空白对照组均为1±0,模型组分别为4.67±0.33、4.50±0.22,实验组分别为2.67±0.21、2.33±0.21;实验组小鼠肝组织细胞凋亡、肝细胞内HMGB1迁移均低于模型组(均P0.001)。结论 HMGB1抗体能改善肝组织的病理损伤,可以保护ConA引起的小鼠肝损伤。
[Abstract]:Objective to study the protective effect of high mobility group protein 1 (HMGB1) antibody on liver injury induced by concanavalin A (ConA) in mice. Methods healthy unpolluted male Balb/c mice were divided into three groups: blank control group (injected with normal saline), model group (injected with ConA) and experimental group (injected with ConA HMGB1 antibody). The eyeballs of the three groups were collected after 6 hours of injection. After centrifugation, serum samples were taken to detect glutamic-pyruvic transaminase (ALT) and HMGB1, for HE staining and Tunel, immunofluorescence. Results the pathological inflammatory reaction of liver tissue in the experimental group was lighter than that in the model group. The serum ALT,HMGB1: blank control group was (52.00 卤8.34) U 鈮，

本文编号：2494542