蛋氨酸脑啡肽小鼠体内抗癌活性初探

发布时间：2019-06-18 08:06

【摘要】：目的研究蛋氨酸脑啡肽(MEK)是否具有抗癌、增加化疗药物疗效和增强或保护免疫功能的作用。方法 BALB/c小鼠40只,右腋皮下接种H22癌细胞,接种24h后随机分为5组,依次为阴性对照组(生理盐水0.2mL/kg)、5-Fu组(10mg/kg)、MEK组(200mg/kg)、5-Fu+MEK联合给药组(10mg/kg+200mg/kg)和阳性对照组(20mg/kg 5-Fu)。腹腔注射受试药品,每日1次,连续给药8d,处死动物。计算肿瘤抑制率、胸腺和脾脏脏器指数以及测定CD3+和CD4+T细胞表达。结果单独给予MEK 200mg/kg或5-Fu 10mg/kg时两组对肿瘤的抑制作用相近,对肿瘤的抑制率分别为75.13%和74.26%;联合给予MEK和5-Fu时,其抑瘤率为88.25%,明显高于单独使用5-Fu。MEK对小鼠脾脏及胸腺无明显抑制作用,与5-Fu合用时能显著减轻5-Fu对免疫脏器胸腺的抑制作用。单独给予MEK 200mg/kg时,能明显提高荷瘤小鼠的CD3+和CD4+T细胞表达,与阴性对照组和5-Fu各剂量组比较差异均有统计学意义(均P0.05)。结论 MEK具有良好的抗肿瘤活性及增强机体免疫功能的作用,与化疗药物合用能增强其疗效同时减轻其毒性,对免疫脏器有保护作用。
[Abstract]:Objective to study whether methionine enkephalin (MEK) has anticancer effect, increase the curative effect of chemotherapeutic drugs and enhance or protect immune function. Methods 40 BALB/c mice were inoculated with H22 cancer cells subcutaneally. after 24 hours of vaccination, they were randomly divided into 5 groups: negative control group (normal saline 0.2mL/kg), 5-Fu group (10mg/kg), MEK group (200mg/kg), 5-Fu MEK combined administration group (10mg/kg 200mg/kg) and positive control group (20mg/kg 5-Fu). The tested drugs were injected intraperitoneally once a day for 8 days, and the animals were killed. The tumor inhibition rate, the index of thymic and spleen organs and the expression of CD3 and CD4 T cells were measured. Results the inhibitory effect of MEK 200mg/kg or 5-Fu 10mg/kg alone on tumor was 75.13% and 74.26% respectively, and the inhibitory rate of MEK and 5-Fu was 88.25%, which was significantly higher than that of 5-Fu.MEK alone on spleen and thymus of mice, and the inhibitory effect of 5-Fu on immune organs was significantly alleviated when combined with 5-Fu. MEK 200mg/kg alone could significantly increase the expression of CD3 and CD4 T cells in tumor-bearing mice, which was significantly different from that in negative control group and 5-Fu group (P 0.05). Conclusion MEK has good antitumor activity and enhances immune function. Combined with chemotherapeutic drugs, it can enhance its curative effect and reduce its toxicity, and has protective effect on immune organs.
【作者单位】：华中科技大学同济医学院附属同济医院药学部;华中科技大学同济医学院药学院药物研究中心;中国医科大学基础医学院免疫学教研室;
【分类号】：R965

【参考文献】