鼻用激素剂量差异对变应性鼻炎小鼠鼻黏膜重塑及MMP-9表达的影响

发布时间：2019-06-24 15:02

【摘要】：目的:探讨不同剂量鼻用激素对变应性鼻炎(AR)小鼠鼻黏膜重塑及基质金属蛋白酶-9(MMP-9)表达的影响。方法:将30只雌性BALB/c小鼠随机分为5组,A组[生理盐水组(NS组)],B组[卵清蛋白组(OVA组)],D1组(低剂量布地奈德组),D2组(中剂量布地奈德组)及D3组(高剂量布地奈德组)。分别接受OVA或NS腹腔注射、鼻部激发。D1、D2及D3组小鼠使用不同剂量的布地奈德鼻喷激素(0.6μg/20g、3.0μg/20g、15.0μg/20g)喷鼻12周,1次/d。分别在4周末及16周末评估小鼠鼻部症状学的变化,并于16周末取小鼠鼻腔组织分别行苏木精-伊红染色、PAS及MT法染色评估其鼻腔黏膜重塑变化。使用ELISA测定小鼠鼻腔黏膜MMP-9的含量。结果:16周末B组小鼠的挠鼻、喷嚏次数及嗜酸粒细胞浸润个数均较A组增多,且出现杯状细胞增多,黏膜下腺体增生、肥大,上皮下纤维化及胶原沉积的变化。0.6μg/20g的布地奈德治疗可以抑制AR小鼠的鼻部症状学变化及嗜酸粒细胞浸润,而对于鼻腔黏膜重塑变化则需要至少3.0μg/20g的鼻喷激素。鼻喷激素可以抑制AR小鼠鼻腔中MMP-9的分泌。结论:高剂量鼻用激素可能通过抑制AR小鼠鼻腔MMP-9的分泌,抑制鼻腔黏膜重塑的发展。
[Abstract]:Objective: To study the effect of different dose of nasal hormone on the expression of matrix metalloproteinase-9 (MMP-9) in allergic rhinitis (AR) mice. Methods:30 female BALB/ c mice were randomly divided into 5 groups, group A[saline group (NS group)], group B[egg albumin group (OVA group)], D1 group (low dose budesonide group), D2 group (middle dose budesonide group) and D3 group (high dose budesonide group). Ova or NS abdominal injection, nasal challenge, respectively. The mice of D1, D2 and D3 used different doses of budesonide nasal spray (0.6. mu.g/20 g, 3.0. mu.g/20 g, 15.0. mu.g/20 g) for 12 weeks and 1/ day. The changes in the nasal symptoms of the mice were assessed at 4 and 16 weekends, respectively. The changes of nasal mucosa remodeling were assessed by hematoxylin-eosin staining, PAS and MT staining, respectively. The content of MMP-9 in nasal mucosa of mice was determined by ELISA. Results: In the 16-week-old group, the number of flexible nose, the number of bleeding and the number of infiltrates of eosinophils was increased in group A, and the increase of goblet-like cells, hyperplasia of the submucosal glands, and hypertrophy were observed. The change of subcutaneous fibrosis and collagen deposition. 0.6. mu.g/20 g of budesonide could inhibit the nasal symptoms of AR mice and the infiltration of eosinophils, while for nasal mucosal remodeling, at least 3.0. m u.g/20 g of nasal spray was required. The nasal spray can inhibit the secretion of MMP-9 in the nasal cavity of AR mice. Conclusion: High-dose nasal hormone may inhibit the development of nasal mucosa remodeling by inhibiting the secretion of MMP-9 in the nasal cavity of AR mice.
【作者单位】：青岛大学附属医院耳鼻咽喉头颈外科;
【基金】：山东省科技发展计划(政策引导类):鼻用皮质类固醇激素剂量对变应性鼻炎大鼠鼻黏膜影响的实验研究(No:2011YD18022)
【分类号】：R96

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