热感赛比斯坦颗粒定性定量方法研究

发布时间：2019-08-01 15:44

【摘要】：目的:建立热感赛比斯坦颗粒定性定量方法。方法:采用薄层色谱法对热感赛比斯坦颗粒中罂粟壳、罂粟子、甘草、破布木果、蜀葵子、黄瓜子、i卙r子和巴旦仁进行定性鉴别;采用高效液相色谱法测定热感赛比斯坦颗粒中甘草苷、甘草酸铵和吗啡的含量。甘草苷和甘草酸铵含量测定色谱条件:采用Agilent TC C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱(0~10 min,19%A;10~15 min,19%A→25%A;15~30 min,25%A→38%A;30~42 min,38%A;42~50 min,38%A→19%A),流速1 m L·min-1,检测波长237 nm,柱温为30℃;吗啡含量测定色谱条件:采用Phenomenex Luna C8(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.5%醋酸铵水溶液(含三乙胺0.8%)(13∶87)为流动相,流速1.0 m L·min-1,检测波长286 nm,柱温30℃。结果:TLC斑点清晰,分离度好,阴性无干扰。甘草苷、甘草酸铵和吗啡质量浓度分别在3.68~73.64、18.62~260.68和5.33~85.28μg·m L-1范围内,与峰面积线性关系良好,r分别为0.999 8、0.999 9和0.999 8);平均回收率(n=6)分别为102.9%、102.2%和99.71%,RSD分别为0.98%、1.4%和1.7%。结论:所建方法可用于热感赛比斯坦颗粒的质量控制。
[Abstract]:Objective: to establish a qualitative and quantitative method for thermal sensitivity Sebstein granules. Methods: the opium poppy shell, poppy seed, Glycyrrhiza uralensis Fisch, rag wooden fruit, solium mandshurica, cucumber seed, I rzi and Padan kernel were identified qualitatively by thin layer chromatography, and the contents of glycyrrhizin, ammonium glycyrrhizinate and morphine in heat sensitive Sebistein granules were determined by high performance liquid chromatography (HPLC), and the contents of glycyrrhizin, ammonium glycyrrhizinate and morphine in heat sensitive Sebistein granules were determined by high performance liquid chromatography (HPLC). The chromatographic conditions for the determination of glycyrrhizin and ammonium glycyrrhizinate were as follows: Agilent TC C18 (250 mm 脳 4.6 mm, 5 渭 m) column, (B) as mobile phase with (A)-0.1% phosphate solution, gradient eluting (0 鈮，

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