行为学训练对局灶性脑缺血大鼠内源性神经干细胞增殖及PS1蛋白表达的影响

发布时间：2019-08-06 12:14

【摘要】：目的检测早老素(Presenilinl,PS1)在局灶性脑缺血大鼠海马组织神经干细胞中的表达及穿梭箱训练的干预作用,探讨影响神经干细胞增殖分化的分子调控机制。方法采用大脑中动脉栓塞(MCAO)制作局灶性脑缺血大鼠模型,用免疫组织化学方法检测海马神经干细胞Brd U、PS1的表达及穿梭箱训练的干预作用。结果随着脑缺血再灌注3 d缺血海马神经元Brd U阳性细胞明显增多,7 d达高峰。PS1反应产物脑缺血再灌注7 d较正常组增多,随缺血再灌注时间的进一步延长逐渐减少,14 d后持续低水平表达。穿梭箱训练后Brd U、PS1蛋白的表达明显增加。结论穿梭箱训练促进神经干细胞的增殖及缺血脑组织PS1的表达,提示穿梭箱训练促进神经干细胞增殖作用可能由PS1信号介导。
[Abstract]:Objective to detect the expression of Presenilinl,PS1 in hippocampal neural stem cells of rats with focal cerebral ischemia and the intervention effect of shuttle box training, and to explore the molecular regulation mechanism of neural stem cells proliferation and differentiation. Methods the rat model of focal cerebral ischemia was established by middle cerebral artery embolism (MCAO). The expression of Brd U and PS1 in hippocampal neural stem cells and the intervention effect of shuttle box training were detected by immunohistochemistry. Results the number of Brd U positive cells in ischemic hippocampal neurons increased significantly with 3 days of cerebral ischemia and reperfusion, and reached the peak at 7 days. PS1 reaction products increased after 7 days of cerebral ischemia and reperfusion, and decreased gradually with the further prolongation of ischemia and reperfusion time, and continued to low expression after 14 days. The expression of PS1 protein increased significantly after shuttle box training. Conclusion shuttle box training promotes the proliferation of neural stem cells and the expression of PS1 in ischemic brain tissue, suggesting that the effect of shuttle box training on the proliferation of neural stem cells may be mediated by PS1 signal.
【作者单位】：沈阳医学院解剖学教研室;沈阳医学院病理学教研室;
【基金】：辽宁省科技厅科学技术计划资助项目(2013020216)
【分类号】：R96

【共引文献】