纳米金和钆掺杂氧化锌量子点纳米载体改善化疗效果
发布时间：2021-08-13 17:22
在过去二十年里,纳米技术因其覆盖了多学科领域并且具有改变世界的巨大潜力,对人类的生活产生了巨大影响。纳米技术是当今世界研究热点之一,在人类日常生活的各个领域都有广泛的应用。大量纳米结构的合成推动了多科学领域的进一步深化研究,这些研究领域包括但不限于半导体器件、传感、太阳能电池、生物医学和纳米药物。尽管已经合成了许多在电子器件制造方面有潜在应用前景的纳米结构,但是在健康医疗方面,纳米颗粒（NPs）仍然面临大量的挑战,例如高毒性等。此外,纳米颗粒（NPs）在细胞或组织内的积聚也是一个有待解决的问题。为此人们开展了复合的纳米颗粒研究,复合纳米颗粒可以同时执行多个任务,在化学分析、催化、储能和药物等领域具有广阔的前景。例如,在医学中,复合纳米颗粒可以同时作为药物载体和诊断探针。在传统的药物输送系统中,采用口服、静脉注射或通过皮肤吸收等模式给药。这些方法都具有血药浓度高、载药/释药能力差、靶点选择性差等缺点,具有长效副作用,危害人类健康。另一方面,慢性疾病如癌症严重威胁着生命,但其治疗在发展中国家和发达国家都仍然存在很多问题,例如肿瘤和癌症治疗专家在癌症诊断和治疗时会遇到多药耐药（MDR）限制化...
【文章来源】：电子科技大学四川省 211工程院校 985工程院校 教育部直属院校
【文章页数】：119 页
【学位级别】：博士
【文章目录】：
摘要
abstract
Chapter 1 Introduction
1.1 General Introduction
1.2 Nanomaterial Systems (NMs)
1.2.1 Core-shell Nanoparticle Systems
1.2.2 Multifunctional Mesoporous Silica (MMS) Nanoparticles
1.2.3 Magnetic Nanoparticles (MNPs)
1.2.4 Quantum Dots
1.2.5 Gold Nanoparticles
1.3 Properties of Nanoparticle
1.3.1 Core Material
1.3.2 Shape and Size
1.3.3 Surface Chemistry
1.4 Objectives
1.5 Thesis Outlines
Chapter 2 Biomedical Applications of Hybrid Nanomaterial
2.1 Introduction
2.2 Cancer: an Overview
2.2.1 Symptoms, Causes, Epidemiology and Cost
2.2.2 Cancer Classification and How it Kills
2.3 Cancer Diagnostics and Treatments in Use Today
2.3.1 Cancer Treatments in Use Today
2.4 Hybrid Nanomaterials Applications in Cancer Treatment
2.5 Uptake and Toxicity of Hybrid Nanomaterial
Chapter 3 Materials, Methods and Characterization Techniques
3.1 Introduction
3.2 Materials
3.2.1 Reagents for Gold Nanoparticles
3.2.2 Reagents for Gadolinium-doped Zinc Oxide Quantum Dots
3.2.3 Surfactant and Chemotherapeutic Agents
3.2.4 Cell Line and Culturing Reagents
3.2.5 Instruments for Analysis
3.3 Method
3.3.1 Synthesis of Gold Nanoparticles
3.3.2 DOX Loading on S1
3.3.3 Nanodrug Formulation; BLM Loading onto S1 and S2 Surfaces
3.3.4 Synthesis of Gadolinium-doped Zinc Oxide (Gd@ZnO) QDs
3.3.5 Functionalization of Gd@ZnO QDs
3.3.6 PEGylation of APTES- Gd@ZnO
3.3.7 Preparation of Nanodrug; DOX Loading
3.4 Functionalization Techniques
3.4.1 Centrifugation Technique
3.4.2 Ultra-sonication
3.4.3 Drug Loading and Release Experiment
3.4.4 Cell Culturing Experiment
3.5 Cell Viability and Cell Imaging
3.6 Statistical Analysis
3.7 Material Characterization
3.7.1 X-ray Diffractometer (XRD)
3.7.2 Dynamic Light Scattering (DLS)
3.7.3 Nanoparticle Tracking Analysis (NTA)
3.7.4 Zeta Potential Measurements
3.7.5 Ultraviolet–visible (UV-Vis) Spectroscopy
3.7.6 Fourier Transform Infrared (FTIR) Spectroscopy
3.7.7 Transmission Electronic Microscopy (TEM)
3.7.8 Laser Scanning Confocal Microscopy (LSCM)
3.8 Conclusion
Chapter 4 Characterization and Functionalization of Hybrid Nanomaterial
4.1 Introduction
4.1.1 Characterization of the Naked and Functionalized Gold Nanoparticles .
4.1.2 Characterization of the Naked and Functionalized Gd@ZnO QDs
4.2 Conclusion
Chapter 5 Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to He La Cells
5.1 Introduction
5.2 Material Characterization
5.3 Flow Cytometric Analysis and LC50, EC50 Determination
5.4 Cellular Internalization and Imaging of He La Cells
5.5 Results and Discussion
5.5.1 Nano-drug Formulations
5.5.2 Nano-drug Characterizations
5.5.2.1 XRD, UV-vis and FTIR Analysis
5.5.2.2 Drug Conjugation Analysis
5.5.2.3 TEM and HRTEM Analysis
5.5.2.4 Nanoparticles Tracking Analysis
5.5.2.5 Cytotoxicity and LC50, EC50 Study
5.5.2.6 Cell Cycle Determination
5.5.2.7 Cellular Internalization and LSCM Imaging
5.5.2.8 NIR Triggered Release Experiment
5.5.2.9 Comparative Analysis
5.6 Conclusion
Chapter 6 Synthesis of Gd@ZnO Quantum Dots and Design of p H-responsive Unique Drug Delivery System
6.1 Introduction
6.2 Results and Discussion
6.2.1 Synthesis of Gd@ZnO QDs
6.2.2 Characterization of Gd@ZnO QDs
6.2.2.1 X-ray diffraction and TEM Analysis
6.2.2.2 DLS Measurements
6.2.2.3 UV-vis and FTIR Analysis
6.2.2.4 Drug Loading/Release Experiment
6.3 Conclusions
Chapter 7 Conclusion and Future Work
7.1 Conclusion
7.2 Advantages and Disadvantages
7.3 Perspective
7.4 Future Work
Acknowledgement
References
Research Results Obtained During the Study for Doctoral Degree
本文编号：3340837
【文章来源】：电子科技大学四川省 211工程院校 985工程院校 教育部直属院校
【文章页数】：119 页
【学位级别】：博士
【文章目录】：
摘要
abstract
Chapter 1 Introduction
1.1 General Introduction
1.2 Nanomaterial Systems (NMs)
1.2.1 Core-shell Nanoparticle Systems
1.2.2 Multifunctional Mesoporous Silica (MMS) Nanoparticles
1.2.3 Magnetic Nanoparticles (MNPs)
1.2.4 Quantum Dots
1.2.5 Gold Nanoparticles
1.3 Properties of Nanoparticle
1.3.1 Core Material
1.3.2 Shape and Size
1.3.3 Surface Chemistry
1.4 Objectives
1.5 Thesis Outlines
Chapter 2 Biomedical Applications of Hybrid Nanomaterial
2.1 Introduction
2.2 Cancer: an Overview
2.2.1 Symptoms, Causes, Epidemiology and Cost
2.2.2 Cancer Classification and How it Kills
2.3 Cancer Diagnostics and Treatments in Use Today
2.3.1 Cancer Treatments in Use Today
2.4 Hybrid Nanomaterials Applications in Cancer Treatment
2.5 Uptake and Toxicity of Hybrid Nanomaterial
Chapter 3 Materials, Methods and Characterization Techniques
3.1 Introduction
3.2 Materials
3.2.1 Reagents for Gold Nanoparticles
3.2.2 Reagents for Gadolinium-doped Zinc Oxide Quantum Dots
3.2.3 Surfactant and Chemotherapeutic Agents
3.2.4 Cell Line and Culturing Reagents
3.2.5 Instruments for Analysis
3.3 Method
3.3.1 Synthesis of Gold Nanoparticles
3.3.2 DOX Loading on S1
3.3.3 Nanodrug Formulation; BLM Loading onto S1 and S2 Surfaces
3.3.4 Synthesis of Gadolinium-doped Zinc Oxide (Gd@ZnO) QDs
3.3.5 Functionalization of Gd@ZnO QDs
3.3.6 PEGylation of APTES- Gd@ZnO
3.3.7 Preparation of Nanodrug; DOX Loading
3.4 Functionalization Techniques
3.4.1 Centrifugation Technique
3.4.2 Ultra-sonication
3.4.3 Drug Loading and Release Experiment
3.4.4 Cell Culturing Experiment
3.5 Cell Viability and Cell Imaging
3.6 Statistical Analysis
3.7 Material Characterization
3.7.1 X-ray Diffractometer (XRD)
3.7.2 Dynamic Light Scattering (DLS)
3.7.3 Nanoparticle Tracking Analysis (NTA)
3.7.4 Zeta Potential Measurements
3.7.5 Ultraviolet–visible (UV-Vis) Spectroscopy
3.7.6 Fourier Transform Infrared (FTIR) Spectroscopy
3.7.7 Transmission Electronic Microscopy (TEM)
3.7.8 Laser Scanning Confocal Microscopy (LSCM)
3.8 Conclusion
Chapter 4 Characterization and Functionalization of Hybrid Nanomaterial
4.1 Introduction
4.1.1 Characterization of the Naked and Functionalized Gold Nanoparticles .
4.1.2 Characterization of the Naked and Functionalized Gd@ZnO QDs
4.2 Conclusion
Chapter 5 Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to He La Cells
5.1 Introduction
5.2 Material Characterization
5.3 Flow Cytometric Analysis and LC50, EC50 Determination
5.4 Cellular Internalization and Imaging of He La Cells
5.5 Results and Discussion
5.5.1 Nano-drug Formulations
5.5.2 Nano-drug Characterizations
5.5.2.1 XRD, UV-vis and FTIR Analysis
5.5.2.2 Drug Conjugation Analysis
5.5.2.3 TEM and HRTEM Analysis
5.5.2.4 Nanoparticles Tracking Analysis
5.5.2.5 Cytotoxicity and LC50, EC50 Study
5.5.2.6 Cell Cycle Determination
5.5.2.7 Cellular Internalization and LSCM Imaging
5.5.2.8 NIR Triggered Release Experiment
5.5.2.9 Comparative Analysis
5.6 Conclusion
Chapter 6 Synthesis of Gd@ZnO Quantum Dots and Design of p H-responsive Unique Drug Delivery System
6.1 Introduction
6.2 Results and Discussion
6.2.1 Synthesis of Gd@ZnO QDs
6.2.2 Characterization of Gd@ZnO QDs
6.2.2.1 X-ray diffraction and TEM Analysis
6.2.2.2 DLS Measurements
6.2.2.3 UV-vis and FTIR Analysis
6.2.2.4 Drug Loading/Release Experiment
6.3 Conclusions
Chapter 7 Conclusion and Future Work
7.1 Conclusion
7.2 Advantages and Disadvantages
7.3 Perspective
7.4 Future Work
Acknowledgement
References
Research Results Obtained During the Study for Doctoral Degree
本文编号：3340837
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/3340837.html
最近更新
教材专著
