乙酸铅和氯化镉暴露对小鼠睾丸支持细胞乳酸水平的影响

发布时间：2018-01-14 18:23

本文关键词：乙酸铅和氯化镉暴露对小鼠睾丸支持细胞乳酸水平的影响　出处：《环境与健康杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的探究乙酸铅、氯化镉单独或联合染毒对小鼠睾丸支持细胞(15P-1细胞)乳酸(LD)水平的影响。方法将15P-1细胞分设对照(空白培养基)组和乙酸铅(0.05~160μmol/L)单独染毒组、氯化镉(0.05~160μmol/L)单独染毒组,染毒24 h后,测定细胞存活率。后续实验分别设对照(空白培养基)组和乙酸铅(1~60μmol/L)单独染毒组、氯化镉(0.5~30μmol/L)单独染毒组及乙酸铅(1、10、20μmol/L)+氯化镉(0.5、5、10μmol/L)联合染毒组,染毒24 h后,测定细胞内葡萄糖摄取量、乳酸脱氢酶(LDH)活力以及细胞内、外LD浓度和p H值。结果与对照组比较,2.5~160μmol/L乙酸铅和2.5~160μmol/L氯化镉分别单独染毒组15P-1细胞存活率均降低(P0.05)。与对照组比较,0.5~10μmol/L氯化镉染毒15P-1细胞内的葡萄糖摄取量较高(P0.05)。与相同浓度乙酸铅+氯化镉染毒组比较,仅0.5μmol/L氯化镉单独染毒组细胞内葡萄糖摄取增加(P0.05)。与对照组比较,1、10μmol/L乙酸铅染毒、各浓度氯化镉染毒及10μmol/L乙酸铅+5μmol/L氯化镉染毒组15P-1细胞内的LD浓度较高,而20~60μmol/L乙酸铅染毒及20μmol/L乙酸铅+10μmol/L氯化镉染毒组15P-1细胞内的LD浓度较低(P0.05);与对照组比较,1~30μmol/L乙酸铅染毒及15、30μmol/L氯化镉染毒15P-1细胞外的LD浓度均较低,而0.5、5μmol/L氯化镉染毒及1μmol/L乙酸铅+0.5μmol/L氯化镉染毒组15P-1细胞外的LD浓度较高(P0.05)。与对照组比较,10~60μmol/L乙酸铅染毒及5~15μmol/L氯化镉染毒15P-1细胞内的p H值较低(P0.05)。与对照组比较,20~60μmol/L乙酸铅染毒及各浓度氯化镉染毒15P-1细胞外的p H值较高(P0.05)。与对照组比较,各浓度乙酸铅+氯化镉染毒组15P-1细胞内、外的p H值均较高(P0.05)。与相同浓度乙酸铅+氯化镉染毒组比较,1、10、20μmol/L乙酸铅单独染毒组及0.5、5、10μmol/L氯化镉染毒组细胞内p H值较高,而1、10、20μmol/L乙酸铅单独染毒组及0.5、10μmol/L氯化镉单独染毒组细胞外p H值较高(P0.05)。与对照组比较,各浓度乙酸铅染毒15P-1细胞内的LDH活力较低,而各浓度氯化镉染毒15P-1细胞内的LDH活力较高(P0.05)。与对照组比较,各浓度乙酸铅+氯化镉染毒组15P-1细胞内LDH活力均较高(P0.05)。与相同浓度乙酸铅+氯化镉染毒组比较,1、10、20μmol/L乙酸铅单独染毒组及5、10μmol/L氯化镉单独染毒组细胞内的LDH活力较高(P0.05)。结论乙酸铅对生精系统的损伤可能通过降低细胞内LDH活力进而抑制细胞内乳酸生成来实现,而氯化镉则可能通过抑制细胞内LD转运到细胞外供给精子发生需要的能量来实现。
[Abstract]:Objective to explore lead acetate. Effects of cadmium chloride alone or in combination on LDL-lactic acid levels in mouse testicular Sertoli cells (Sertoli cells 15P-1). Methods 15P-1 cells were divided into control (blank medium) group and lead acetate (Pb) acetate group. 0. 05 渭 mol / L) alone. The cadmium chloride group (0.05 渭 mol / L) was exposed to cadmium chloride alone for 24 h. The cell survival rate was measured. The following experiments were divided into two groups: control group (blank medium) and lead acetate group (60 渭 mol / L) alone. Cadmium chloride (0.5 ~ 30 渭 mol 路L ~ (-1)) and lead acetate (10 ~ 10 ~ (20 渭 mol / L)) and cadmium chloride (0.5 ~ 5 渭 mol 路L ~ (-1)) combined with 10 渭 mol 路L ~ (-1 / L) group. After exposure for 24 h, the intracellular glucose intake, the activity of lactate dehydrogenase (LDH), the intracellular and extracellular LD concentration and pH value were measured, and the results were compared with those in the control group. The survival rate of 15P-1 cells in 2.5 ~ 160 渭 mol/L lead acetate group and 2.5 ~ 160 渭 mol/L cadmium chloride group was significantly lower than that in control group. The glucose uptake in 15P-1 cells exposed to 0.5 渭 mol/L cadmium chloride was higher than that in the same concentration of lead acetate and cadmium chloride group. Only 0.5 渭 mol/L cadmium chloride alone increased the intracellular glucose uptake of P0.05G, and compared with the control group, 10 渭 mol/L lead acetate was exposed to lead acetate. LD concentrations in 15P-1 cells were higher in cadmium chloride and 10 渭 mol/L lead acetate (5 渭 mol/L) groups. The LD concentration in 15P-1 cells of 20 渭 mol/L lead acetate and 20 渭 mol/L lead acetate 10 渭 mol/L cadmium chloride group was lower than that of 20 渭 mol/L lead acetate group. Compared with the control group, the concentration of LD in 15P-1 cells exposed to 30 渭 mol/L lead acetate and 1530 渭 mol/L cadmium chloride was lower than that in the control group. The LD concentration in 15P-1 cells of 5 渭 mol/L cadmium chloride group and 1 渭 mol/L lead acetate 0.5 渭 mol/L cadmium chloride group was higher than that of control group. The pH value of 15P-1 cells exposed to 10 ~ 60 渭 mol/L lead acetate and 5 ~ 15 渭 mol/L cadmium chloride was lower than that of control group. The pH value of 15P-1 cells exposed to 20 ~ 60 渭 mol/L lead acetate and cadmium chloride was higher than that of control group. Compared with the same concentration of lead acetate cadmium chloride group, the pH value of 15 P-1 cells was higher than that of the same concentration lead acetate cadmium chloride group. The intracellular pH value of 20 渭 mol/L lead acetate alone group and 0.5 渭 mol/L 10 渭 mol/L cadmium chloride group was higher than that of 1 渭 mol/L group. The extracellular pH value of 20 渭 mol/L lead acetate alone group and 0.5 渭 mol/L 10 渭 mol/L cadmium chloride group was higher than that of control group. The LDH activity in 15P-1 cells exposed to lead acetate was lower than that in the control group, while the LDH activity in 15P-1 cells exposed to cadmium chloride was higher than that in the control group. The activity of LDH in 15P-1 cells was higher in each concentration of lead acetate and cadmium chloride group than that in the same concentration of lead acetate and cadmium chloride group. 20 渭 mol/L lead acetate alone and 5. The intracellular LDH activity of 10 渭 mol/L cadmium chloride alone group was higher than that of P0.05 group. Conclusion the damage of lead acetate to spermatogenic system may be achieved by reducing the activity of LDH and inhibiting the production of lactic acid. Cadmium chloride may be achieved by inhibiting intracellular LD transport to the extracellular supply of energy needed for spermatogenesis.
【作者单位】：武汉大学健康学院;
【基金】：国家博士后科学基金(2015M582279) 国家自然科学基金(81773471)
【分类号】：R114
【正文快照】： 环境重金属铅和镉进入机体后与组织器官有较高的亲和性,且蓄积后不易排除。近年来,关于铅、镉暴露导致的男性不育等问题引起了广泛的关注[1],但其作用机制仍不十分明确。睾丸是自然状态下相对缺氧器官,睾丸支持细胞(Sertoli cell,SC)可通过糖酵解作用生成乳酸,同时,为生精细胞

【相似文献】