鸡枞菌多糖对急性酒精肝损伤小鼠超微病理结构及ADH2、ALDH2 mRNA表达的影响

发布时间：2018-01-15 13:22

本文关键词：鸡枞菌多糖对急性酒精肝损伤小鼠超微病理结构及ADH2、ALDH2 mRNA表达的影响　出处：《食品科学》2015年05期 　论文类型：期刊论文

【摘要】：目的:从小鼠肝脏超微病理结构及酒精代谢相关酶乙醇脱氢酶2(alcohol dehydrogenase 2,ADH2)、乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)的m RNA表达方面研究鸡枞菌多糖(refined polysaccharides from Termitomyces albuminosus,RPTA)对酒精所致小鼠急性肝损伤的保护作用。方法:小鼠被随机分为空白对照组、模型对照组、药物对照组(联苯双酯组,150 mg/(kg·d))、RPTA各剂量组(1 00、200、400 mg/(kg·d)),连续灌胃30 d,空白对照组灌胃等量生理盐水。第31天,给予50%乙醇(12 m L/kg)建立动物急性肝损伤模型。12 h后处死,取小鼠肝脏分别观察肝组织超微结构变化,并采用荧光实时定量聚合酶链式反应(real time polymerase chain reaction,real time-PCR)法检测肝脏ADH2和ALDH2的m RNA表达。结果:超微结构观察结果表明,模型对照组小鼠肝脏细胞内可见大量脂滴,细胞核呈不规则形态,局部核膜凹陷严重,线粒体严重变形,线粒体嵴模糊,内质网严重肿胀,核糖体脱落;而RPTA小鼠肝细胞内上述病变有所改善,尤以高剂量组最佳。Real time-PCR结果表明,与空白对照组相比,模型对照组ADH2和ALDH2的m RNA表达量降低,而RPTA组随着剂量的增加ADH2和ALDH2 m RNA的表达逐渐升高。结论:RPTA可以上调ADH2和ALDH2 m RNA的表达,具有改善小鼠酒精性肝损伤状况的作用。
[Abstract]:Objective: to study the ultrastructure of liver and alcohol metabolism related enzyme alcohol dehydrogenase (alcohol dehydrogenase 2alcohol dehydrogenase 2 ADH2) in mice. Aldehyde dehydrogenase 2aldehyde dehydrogenase 2. Study on m RNA expression of ALDH2. Refined polysaccharides from Termitomyces albuminosus. Methods: mice were randomly divided into blank control group, model control group and drug control group (biphenyl diester group). 1 00,200,400 mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1) mg/(kg 路d ~ (-1). The blank control group was given the same amount of normal saline. On the 31st day, 50% ethanol was given for 12 mL / kg) to establish the animal model of acute liver injury. The ultrastructural changes of liver tissue were observed in mice. The real time polymerase chain reaction was used for real-time quantitative polymerase chain reaction. The expression of m RNA in liver ADH2 and ALDH2 was detected by real time-PCR method. In the model control group, a large number of lipid droplets were found in the liver cells, the nucleus was irregular, the local nuclear membrane was depressed seriously, the mitochondria was deformed seriously, the mitochondrial ridge was blurred, the endoplasmic reticulum was seriously swollen and ribosome fell off. However, the above pathological changes in hepatocytes of RPTA mice were improved, especially in the high dose group. Real time-PCR results showed that compared with the blank control group. The expression of m RNA in ADH2 and ALDH2 was decreased in the model control group. However, the expression of ADH2 and ALDH2 m RNA increased gradually with the increase of dose in RPTA group. Conclusion the expression of ADH2 and ALDH2 m RNA can be upregulated by RPTA. It can improve the status of alcoholic liver injury in mice.
【作者单位】：南京师范大学金陵女子学院;南京师范大学生命科学学院;
【基金】：江苏省高校自然科学基础研究项目(10KJD550003)
【分类号】：R151
【正文快照】： 酒精性肝病(alcoholic liver disease,ALD)是由于饮酒过量引起的中毒性肝脏疾病,重度饮酒者中80%以上有一定程度的脂肪肝,其中10%~35%可发展为酒精性肝炎,而10%~20%将发展为肝硬化,尤其是近年来随着酒精消费的增加ALD的发病率呈逐年上升趋势[1],ALD已经成为当今全世界范围内倍

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