低剂量电离辐射、一氧化碳、苯和噪声复合作用下大鼠血清的代谢组学研究

发布时间：2018-01-15 17:32

本文关键词：低剂量电离辐射、一氧化碳、苯和噪声复合作用下大鼠血清的代谢组学研究　出处：《解放军医学杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的探讨低剂量电离辐射、一氧化碳、苯和噪声有害环境因素复合作用对大鼠血清代谢产物的影响,探讨复合环境因素导致机体损伤的机制。方法健康成年SD大鼠16只,随机分为正常对照组(不做任何处理)和实验组(每日接受低剂量电离辐射、一氧化碳、苯和噪声的复合作用,共7d),每组8只。实验结束时腹主动脉取血,分离大鼠血清,采用核磁共振氢谱(1H NMR)仪检测两组大鼠血清样本,得到代谢指纹图谱,采用主成分分析(PCA)和正交信号校正-偏最小二乘(OSC-PLS)法进行模式识别,并通过SIMCA-P软件鉴别两组代谢产物谱的差异。结果大鼠血清1H NMR图谱分析显示对照组和实验组的代谢谱存在明显差异,OSC-PLS分析显示两组代谢产物各自聚集,分布区域明显分开。与对照组比较,实验组血清中脂类、高密度脂蛋白、甘氨酸/葡萄糖、N-乙酰糖蛋白1、N-乙酰糖蛋白2、磷脂酰胆碱和不饱和脂肪酸含量增加,乳酸、苏氨酸/脂质、丙氨酸、肌酸、甘磷酸胆碱/氧化三甲胺、低密度脂蛋白/高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白/低密度脂蛋白、极低密度脂蛋白和饱和脂肪酸含量下降。结论低剂量电离辐射、一氧化碳、苯和噪声有害环境因素复合作用使大鼠血清代谢产物发生变化,主要涉及免疫功能、肾功能和能量代谢。基于核磁共振的代谢组学方法可用于研究多种有害环境因素的复合生物效应,具有较好的应用前景。
[Abstract]:Objective to evaluate the effect of low doses of ionizing radiation, carbon monoxide, benzene and noise is harmful effect of the combination of environmental factors on rat blood metabolites in the Qing Dynasty, to explore the mechanism of composite environmental factors cause body injury. Methods 16 healthy adult SD rats, were randomly divided into normal control group (without any treatment) and experimental group (received daily low doses of ionizing radiation, carbon monoxide, compound, benzene and noise of 7D), 8 rats in each group. At the end of the experiment, blood from the abdominal aorta, isolated from rat serum by nuclear magnetic resonance spectroscopy (1H NMR) in serum samples of two groups of rats were detected, get metabolic fingerprint, using principal component analysis (PCA orthogonal signal correction) and partial least squares (OSC-PLS) method for pattern recognition, the difference through the SIMCA-P software to identify the two group metabolite spectrum. Results the serum 1H NMR spectrum analysis showed that the experimental group and the control group have obvious metabolic profiles The difference, OSC-PLS analysis showed that two groups of metabolites each aggregation, distinct regional distributions separately. Compared with the control group, the serum lipids, high density lipoprotein, glucose / glycine, acetyl N- glycoprotein 1, N- acetyl glycoprotein 2, increased phosphatidylcholine and unsaturated fatty acid content of lactic acid, threonine / lipid. Alanine, creatine, choline alfoscerate / trimethylamineoxide, LDL / HDL, LDL, VLDL / LDL decreased VLDL and saturated fatty acids. Conclusion low dose ionizing radiation, carbon monoxide, benzene and noise harmful compound effect the environmental factors of rat serum metabolic product changes, mainly related to immune function, renal function and metabolic energy metabolism. The NMR method can be used for composite biological effects of a variety of harmful environmental factors based on, It has a good application prospect.

【作者单位】：海军医学研究所;
【基金】：全军后勤科研计划项目(AHJ09J012) 总后科技攻关项目(2009183006)~~
【分类号】：R114
【正文快照】： 某些特殊作业环境中同时存在多种有害理化因素,这些因素复合作用于人体会对健康造成一定影响。动物实验表明,一些复合环境因素产生的生物效应较为复杂,既有拮抗作用,也存在协同作用[1-4],但上述研究主要集中在动物组织器官病理变化、生理生化指标变化及细胞水平的改变方面,对

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